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MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer

BACKGROUND: To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. METHODS: In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistoch...

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Autores principales: Vos, Maria Caroline, Hollemans, Eva, Ezendam, Nicole, Feijen, Harry, Boll, Dorry, Pijlman, Brenda, van der Putten, Hans, Klinkhamer, Paul, van Kuppevelt, Toin H., van der Wurff, Anneke A. M., Massuger, Leon F. A. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010680/
https://www.ncbi.nlm.nih.gov/pubmed/27590006
http://dx.doi.org/10.1186/s13048-016-0262-7
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author Vos, Maria Caroline
Hollemans, Eva
Ezendam, Nicole
Feijen, Harry
Boll, Dorry
Pijlman, Brenda
van der Putten, Hans
Klinkhamer, Paul
van Kuppevelt, Toin H.
van der Wurff, Anneke A. M.
Massuger, Leon F. A. G.
author_facet Vos, Maria Caroline
Hollemans, Eva
Ezendam, Nicole
Feijen, Harry
Boll, Dorry
Pijlman, Brenda
van der Putten, Hans
Klinkhamer, Paul
van Kuppevelt, Toin H.
van der Wurff, Anneke A. M.
Massuger, Leon F. A. G.
author_sort Vos, Maria Caroline
collection PubMed
description BACKGROUND: To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. METHODS: In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed. RESULTS: Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05). In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters. CONCLUSION: The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found.
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spelling pubmed-50106802016-09-04 MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer Vos, Maria Caroline Hollemans, Eva Ezendam, Nicole Feijen, Harry Boll, Dorry Pijlman, Brenda van der Putten, Hans Klinkhamer, Paul van Kuppevelt, Toin H. van der Wurff, Anneke A. M. Massuger, Leon F. A. G. J Ovarian Res Research BACKGROUND: To investigate the expression of MMP-14 and CD44 as well as epithelial-to-mesenchymal transition(EMT)-like changes in ovarian cancer and to determine correlations with clinical outcome. METHODS: In 97 patients with ovarian cancer, MMP-14 and CD44 expression as determined by immunohistochemistry was investigated in relation to EMT-like changes. To determine this, immunohistochemical staining of E-cadherin and vimentin was performed. RESULTS: Patients with expression of both MMP-14 and CD44 in their tumors had a poor prognosis despite complete debulking. Serous histology in advanced-stage tumors (FIGO IIB-IV) correlated with CD44 (rho .286, p < 0.01). Also, CD44 correlated with percentage vimentin expression (rho .217, p < 0.05). In logistic regression analysis with complete debulking as the outcome parameter, CD44 expression was found to be significant (OR 3,571 (95 % Confidence Interval 1,112–11,468) p = 0.032), though this was not the case for MMP-14 and EMT parameters. CONCLUSION: The subgroup of patients with double expression of MMP-14 and CD44 had a poor prognosis despite complete debulking. Serous subtype in advanced-stage patients and CD44 expression were found to be correlated with vimentin expression, and CD44 expression was found to be significantly correlated with complete debulking. However, a significant correlation between EMT and clinical parameters was not found. BioMed Central 2016-09-02 /pmc/articles/PMC5010680/ /pubmed/27590006 http://dx.doi.org/10.1186/s13048-016-0262-7 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Vos, Maria Caroline
Hollemans, Eva
Ezendam, Nicole
Feijen, Harry
Boll, Dorry
Pijlman, Brenda
van der Putten, Hans
Klinkhamer, Paul
van Kuppevelt, Toin H.
van der Wurff, Anneke A. M.
Massuger, Leon F. A. G.
MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title_full MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title_fullStr MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title_full_unstemmed MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title_short MMP-14 and CD44 in Epithelial-to-Mesenchymal Transition (EMT) in ovarian cancer
title_sort mmp-14 and cd44 in epithelial-to-mesenchymal transition (emt) in ovarian cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010680/
https://www.ncbi.nlm.nih.gov/pubmed/27590006
http://dx.doi.org/10.1186/s13048-016-0262-7
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