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Data on screening and identification of genetically modified papaya in food supplements

This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with...

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Detalles Bibliográficos
Autores principales: Prins, Theo W., Scholtens, Ingrid M.J., Bak, Arno W., van Dijk, Jeroen P., Voorhuijzen, Marleen M., Laurensse, Emile J., Kok, Esther J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011157/
https://www.ncbi.nlm.nih.gov/pubmed/27626052
http://dx.doi.org/10.1016/j.dib.2016.08.028
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author Prins, Theo W.
Scholtens, Ingrid M.J.
Bak, Arno W.
van Dijk, Jeroen P.
Voorhuijzen, Marleen M.
Laurensse, Emile J.
Kok, Esther J.
author_facet Prins, Theo W.
Scholtens, Ingrid M.J.
Bak, Arno W.
van Dijk, Jeroen P.
Voorhuijzen, Marleen M.
Laurensse, Emile J.
Kok, Esther J.
author_sort Prins, Theo W.
collection PubMed
description This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with targets that are putatively present in genetically modified (GM) papaya was used as a first screening to narrow down the vast array of candidates. The combination of elements P-nos and nptII was further confirmed by amplification and subsequent sequencing of the P-nos/nptII construct. Next, presence of the candidate GM papayas 16-0-1 and 18-2-4 were investigated by amplification and sequencing of event-spanning regions on the left and right border. This data article reports the Cq values for GM elements, the nucleotide sequence of the P-nos/nptII construct and the presence of GM papaya events 18-2-4 and/or 16-0-1 in five samples that were randomly sampled to be analysed in the framework of the official Dutch GMO monitoring program for food.
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spelling pubmed-50111572016-09-13 Data on screening and identification of genetically modified papaya in food supplements Prins, Theo W. Scholtens, Ingrid M.J. Bak, Arno W. van Dijk, Jeroen P. Voorhuijzen, Marleen M. Laurensse, Emile J. Kok, Esther J. Data Brief Data Article This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al., 2016) [1]. Quantitative real-time PCR (qPCR) with targets that are putatively present in genetically modified (GM) papaya was used as a first screening to narrow down the vast array of candidates. The combination of elements P-nos and nptII was further confirmed by amplification and subsequent sequencing of the P-nos/nptII construct. Next, presence of the candidate GM papayas 16-0-1 and 18-2-4 were investigated by amplification and sequencing of event-spanning regions on the left and right border. This data article reports the Cq values for GM elements, the nucleotide sequence of the P-nos/nptII construct and the presence of GM papaya events 18-2-4 and/or 16-0-1 in five samples that were randomly sampled to be analysed in the framework of the official Dutch GMO monitoring program for food. Elsevier 2016-08-20 /pmc/articles/PMC5011157/ /pubmed/27626052 http://dx.doi.org/10.1016/j.dib.2016.08.028 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Prins, Theo W.
Scholtens, Ingrid M.J.
Bak, Arno W.
van Dijk, Jeroen P.
Voorhuijzen, Marleen M.
Laurensse, Emile J.
Kok, Esther J.
Data on screening and identification of genetically modified papaya in food supplements
title Data on screening and identification of genetically modified papaya in food supplements
title_full Data on screening and identification of genetically modified papaya in food supplements
title_fullStr Data on screening and identification of genetically modified papaya in food supplements
title_full_unstemmed Data on screening and identification of genetically modified papaya in food supplements
title_short Data on screening and identification of genetically modified papaya in food supplements
title_sort data on screening and identification of genetically modified papaya in food supplements
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011157/
https://www.ncbi.nlm.nih.gov/pubmed/27626052
http://dx.doi.org/10.1016/j.dib.2016.08.028
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