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A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops
Importance. Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature. Objective. This study sought to evaluate the total reactive antioxidant potential (TR...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011221/ https://www.ncbi.nlm.nih.gov/pubmed/27635188 http://dx.doi.org/10.1155/2016/9780193 |
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author | Gus, Patrícia Ioschpe Marinho, Diane Zelanis, Samira Belló-Klein, Adriane Locatelli, Claudete Nicola, Felipe Kunzler, Ana Laura Fernandes, Tania Regina Gatelli Carraro, Cristina Campos Barbosa, Luciene |
author_facet | Gus, Patrícia Ioschpe Marinho, Diane Zelanis, Samira Belló-Klein, Adriane Locatelli, Claudete Nicola, Felipe Kunzler, Ana Laura Fernandes, Tania Regina Gatelli Carraro, Cristina Campos Barbosa, Luciene |
author_sort | Gus, Patrícia Ioschpe |
collection | PubMed |
description | Importance. Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature. Objective. This study sought to evaluate the total reactive antioxidant potential (TRAP) and concentration of reactive oxygen species (ROS) in samples of 50% AS eye drops and their correlations with the demographic characteristics and lifestyle habits of patients with ocular surface disease and healthy controls. Design. This was a case-control study with a 3-month follow-up period. Participants. 16 patients with severe dry eye disease of different etiologies and 17 healthy controls matched by age, gender, and race were included. Results. TRAP and ROS were detected at all evaluated times. There were no differences in the mean ROS (p = 0.429) or TRAP (p = 0.475) levels between cases and controls. No statistically significant differences in the concentrations of ROS or TRAPs were found at 0, 15, or 30 days (p for ROS = 0.087 and p for TRAP = 0.93). Neither the demographic characteristics nor the lifestyle habits were correlated with the oxidative balance of the 50% AS eye drops. Conclusions and Relevance. Both fresh and frozen 50% AS eye drops present antioxidant capacities and ROS in an apparently stable balance. Moreover, patients with ocular surface disease and normal controls produce equivalent AS eye drops in terms of oxidative properties. |
format | Online Article Text |
id | pubmed-5011221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50112212016-09-15 A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops Gus, Patrícia Ioschpe Marinho, Diane Zelanis, Samira Belló-Klein, Adriane Locatelli, Claudete Nicola, Felipe Kunzler, Ana Laura Fernandes, Tania Regina Gatelli Carraro, Cristina Campos Barbosa, Luciene Oxid Med Cell Longev Research Article Importance. Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature. Objective. This study sought to evaluate the total reactive antioxidant potential (TRAP) and concentration of reactive oxygen species (ROS) in samples of 50% AS eye drops and their correlations with the demographic characteristics and lifestyle habits of patients with ocular surface disease and healthy controls. Design. This was a case-control study with a 3-month follow-up period. Participants. 16 patients with severe dry eye disease of different etiologies and 17 healthy controls matched by age, gender, and race were included. Results. TRAP and ROS were detected at all evaluated times. There were no differences in the mean ROS (p = 0.429) or TRAP (p = 0.475) levels between cases and controls. No statistically significant differences in the concentrations of ROS or TRAPs were found at 0, 15, or 30 days (p for ROS = 0.087 and p for TRAP = 0.93). Neither the demographic characteristics nor the lifestyle habits were correlated with the oxidative balance of the 50% AS eye drops. Conclusions and Relevance. Both fresh and frozen 50% AS eye drops present antioxidant capacities and ROS in an apparently stable balance. Moreover, patients with ocular surface disease and normal controls produce equivalent AS eye drops in terms of oxidative properties. Hindawi Publishing Corporation 2016 2016-08-21 /pmc/articles/PMC5011221/ /pubmed/27635188 http://dx.doi.org/10.1155/2016/9780193 Text en Copyright © 2016 Patrícia Ioschpe Gus et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gus, Patrícia Ioschpe Marinho, Diane Zelanis, Samira Belló-Klein, Adriane Locatelli, Claudete Nicola, Felipe Kunzler, Ana Laura Fernandes, Tania Regina Gatelli Carraro, Cristina Campos Barbosa, Luciene A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title | A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title_full | A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title_fullStr | A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title_full_unstemmed | A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title_short | A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops |
title_sort | case-control study on the oxidative balance of 50% autologous serum eye drops |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011221/ https://www.ncbi.nlm.nih.gov/pubmed/27635188 http://dx.doi.org/10.1155/2016/9780193 |
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