Cargando…

Impact of Helicobacter pylori on the healing process of the gastric barrier

AIM: To determine the impact of selected well defined Helicobacter pylori (H. pylori) antigens on gastric barrier cell turnover. METHODS: In this study, using two cellular models of gastric epithelial cells and fibroblasts, we have focused on exploring the effects of well defined H. pylori soluble c...

Descripción completa

Detalles Bibliográficos
Autores principales: Mnich, Eliza, Kowalewicz-Kulbat, Magdalena, Sicińska, Paulina, Hinc, Krzysztof, Obuchowski, Michał, Gajewski, Adrian, Moran, Anthony P, Chmiela, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011668/
https://www.ncbi.nlm.nih.gov/pubmed/27672275
http://dx.doi.org/10.3748/wjg.v22.i33.7536
_version_ 1782451869581312000
author Mnich, Eliza
Kowalewicz-Kulbat, Magdalena
Sicińska, Paulina
Hinc, Krzysztof
Obuchowski, Michał
Gajewski, Adrian
Moran, Anthony P
Chmiela, Magdalena
author_facet Mnich, Eliza
Kowalewicz-Kulbat, Magdalena
Sicińska, Paulina
Hinc, Krzysztof
Obuchowski, Michał
Gajewski, Adrian
Moran, Anthony P
Chmiela, Magdalena
author_sort Mnich, Eliza
collection PubMed
description AIM: To determine the impact of selected well defined Helicobacter pylori (H. pylori) antigens on gastric barrier cell turnover. METHODS: In this study, using two cellular models of gastric epithelial cells and fibroblasts, we have focused on exploring the effects of well defined H. pylori soluble components such as glycine acid extract antigenic complex (GE), subunit A of urease (UreA), cytotoxin associated gene A protein (CagA) and lipopolysaccharide (LPS) on cell turnover by comparing the wound healing capacity of the cells in terms of their proliferative and metabolic activity as well as cell cycle distribution. Toxic effects of H. pylori components have been assessed in an association with damage to cell nuclei and inhibition of signal transducer and activator of transcription 3 (STAT3) phosphorylation. RESULTS: We showed that H. pylori GE, CagA and UreA promoted regeneration of epithelial cells and fibroblasts, which is necessary for effective tissue healing. However, in vivo increased proliferative activity of these cells may constitute an increased risk of gastric neoplasia. In contrast, H. pylori LPS showed a dose-dependent influence on the process of wound healing. At a low concentration (1 ng/mL) H. pylori LPS accelerated of healing epithelial cells, which was linked to significantly enhanced cell proliferation and MTT reduction as well as lack of alterations in cell cycle and downregulation of epidermal growth factor (EGF) production as well as cell nuclei destruction. By comparison, H. pylori LPS at a high concentration (25 ng/mL) inhibited the process of wound repair, which was related to diminished proliferative activity of the cells, cell cycle arrest, destruction of cell nuclei and downregulation of the EGF/STAT3 signalling pathway. CONCLUSION: In vivo H. pylori LPS driven effects might lead to the maintenance of chronic inflammatory response and pathological disorders on the level of the gastric mucosal barrier.
format Online
Article
Text
id pubmed-5011668
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Baishideng Publishing Group Inc
record_format MEDLINE/PubMed
spelling pubmed-50116682016-09-26 Impact of Helicobacter pylori on the healing process of the gastric barrier Mnich, Eliza Kowalewicz-Kulbat, Magdalena Sicińska, Paulina Hinc, Krzysztof Obuchowski, Michał Gajewski, Adrian Moran, Anthony P Chmiela, Magdalena World J Gastroenterol Basic Study AIM: To determine the impact of selected well defined Helicobacter pylori (H. pylori) antigens on gastric barrier cell turnover. METHODS: In this study, using two cellular models of gastric epithelial cells and fibroblasts, we have focused on exploring the effects of well defined H. pylori soluble components such as glycine acid extract antigenic complex (GE), subunit A of urease (UreA), cytotoxin associated gene A protein (CagA) and lipopolysaccharide (LPS) on cell turnover by comparing the wound healing capacity of the cells in terms of their proliferative and metabolic activity as well as cell cycle distribution. Toxic effects of H. pylori components have been assessed in an association with damage to cell nuclei and inhibition of signal transducer and activator of transcription 3 (STAT3) phosphorylation. RESULTS: We showed that H. pylori GE, CagA and UreA promoted regeneration of epithelial cells and fibroblasts, which is necessary for effective tissue healing. However, in vivo increased proliferative activity of these cells may constitute an increased risk of gastric neoplasia. In contrast, H. pylori LPS showed a dose-dependent influence on the process of wound healing. At a low concentration (1 ng/mL) H. pylori LPS accelerated of healing epithelial cells, which was linked to significantly enhanced cell proliferation and MTT reduction as well as lack of alterations in cell cycle and downregulation of epidermal growth factor (EGF) production as well as cell nuclei destruction. By comparison, H. pylori LPS at a high concentration (25 ng/mL) inhibited the process of wound repair, which was related to diminished proliferative activity of the cells, cell cycle arrest, destruction of cell nuclei and downregulation of the EGF/STAT3 signalling pathway. CONCLUSION: In vivo H. pylori LPS driven effects might lead to the maintenance of chronic inflammatory response and pathological disorders on the level of the gastric mucosal barrier. Baishideng Publishing Group Inc 2016-09-07 2016-09-07 /pmc/articles/PMC5011668/ /pubmed/27672275 http://dx.doi.org/10.3748/wjg.v22.i33.7536 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Mnich, Eliza
Kowalewicz-Kulbat, Magdalena
Sicińska, Paulina
Hinc, Krzysztof
Obuchowski, Michał
Gajewski, Adrian
Moran, Anthony P
Chmiela, Magdalena
Impact of Helicobacter pylori on the healing process of the gastric barrier
title Impact of Helicobacter pylori on the healing process of the gastric barrier
title_full Impact of Helicobacter pylori on the healing process of the gastric barrier
title_fullStr Impact of Helicobacter pylori on the healing process of the gastric barrier
title_full_unstemmed Impact of Helicobacter pylori on the healing process of the gastric barrier
title_short Impact of Helicobacter pylori on the healing process of the gastric barrier
title_sort impact of helicobacter pylori on the healing process of the gastric barrier
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5011668/
https://www.ncbi.nlm.nih.gov/pubmed/27672275
http://dx.doi.org/10.3748/wjg.v22.i33.7536
work_keys_str_mv AT mnicheliza impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT kowalewiczkulbatmagdalena impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT sicinskapaulina impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT hinckrzysztof impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT obuchowskimichał impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT gajewskiadrian impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT morananthonyp impactofhelicobacterpylorionthehealingprocessofthegastricbarrier
AT chmielamagdalena impactofhelicobacterpylorionthehealingprocessofthegastricbarrier