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The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela

Qualitative expression of dissimilative sulfite reductase (dsrA), a key gene in sulfate reduction, and sulfide:quinone oxidoreductase (sqr), a key gene in sulfide oxidation was investigated. Neither of the two could be amplified from mRNA retrieved with Niskin bottles but were amplified from mRNA re...

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Autores principales: Rodriguez-Mora, Maria J., Edgcomb, Virginia P., Taylor, Craig, Scranton, Mary I., Taylor, Gordon T., Chistoserdov, Andrei Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012083/
https://www.ncbi.nlm.nih.gov/pubmed/27651847
http://dx.doi.org/10.2174/1874285801610010140
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author Rodriguez-Mora, Maria J.
Edgcomb, Virginia P.
Taylor, Craig
Scranton, Mary I.
Taylor, Gordon T.
Chistoserdov, Andrei Y.
author_facet Rodriguez-Mora, Maria J.
Edgcomb, Virginia P.
Taylor, Craig
Scranton, Mary I.
Taylor, Gordon T.
Chistoserdov, Andrei Y.
author_sort Rodriguez-Mora, Maria J.
collection PubMed
description Qualitative expression of dissimilative sulfite reductase (dsrA), a key gene in sulfate reduction, and sulfide:quinone oxidoreductase (sqr), a key gene in sulfide oxidation was investigated. Neither of the two could be amplified from mRNA retrieved with Niskin bottles but were amplified from mRNA retrieved by the Deep SID. The sqr and sqr-like genes retrieved from the Cariaco Basin were related to the sqr genes from a Bradyrhizobium sp., Methylomicrobium alcaliphilum, Sulfurovum sp. NBC37-1, Sulfurimonas autotrophica, Thiorhodospira sibirica and Chlorobium tepidum. The dsrA gene sequences obtained from the redoxcline of the Cariaco Basin belonged to chemoorganotrophic and chemoautotrophic sulfate and sulfur reducers belonging to the class Deltaproteobacteria (phylum Proteobacteria) and the order Clostridiales (phylum Firmicutes).
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spelling pubmed-50120832016-09-20 The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela Rodriguez-Mora, Maria J. Edgcomb, Virginia P. Taylor, Craig Scranton, Mary I. Taylor, Gordon T. Chistoserdov, Andrei Y. Open Microbiol J Article Qualitative expression of dissimilative sulfite reductase (dsrA), a key gene in sulfate reduction, and sulfide:quinone oxidoreductase (sqr), a key gene in sulfide oxidation was investigated. Neither of the two could be amplified from mRNA retrieved with Niskin bottles but were amplified from mRNA retrieved by the Deep SID. The sqr and sqr-like genes retrieved from the Cariaco Basin were related to the sqr genes from a Bradyrhizobium sp., Methylomicrobium alcaliphilum, Sulfurovum sp. NBC37-1, Sulfurimonas autotrophica, Thiorhodospira sibirica and Chlorobium tepidum. The dsrA gene sequences obtained from the redoxcline of the Cariaco Basin belonged to chemoorganotrophic and chemoautotrophic sulfate and sulfur reducers belonging to the class Deltaproteobacteria (phylum Proteobacteria) and the order Clostridiales (phylum Firmicutes). Bentham Open 2016-08-31 /pmc/articles/PMC5012083/ /pubmed/27651847 http://dx.doi.org/10.2174/1874285801610010140 Text en © Rodriguez-Mora et al.; Licensee Bentham Open https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Rodriguez-Mora, Maria J.
Edgcomb, Virginia P.
Taylor, Craig
Scranton, Mary I.
Taylor, Gordon T.
Chistoserdov, Andrei Y.
The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title_full The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title_fullStr The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title_full_unstemmed The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title_short The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela
title_sort diversity of sulfide oxidation and sulfate reduction genes expressed by the bacterial communities of the cariaco basin, venezuela
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012083/
https://www.ncbi.nlm.nih.gov/pubmed/27651847
http://dx.doi.org/10.2174/1874285801610010140
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