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Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)

Asian seabass (Lates calcarifer) is a food fish of increasing aquaculture importance. In order to improve our understanding on the digestive system and feeding of this species, morphological and histological features of the gut were studied. Morphologically, the Asian seabass gut is defined by a sho...

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Autores principales: Purushothaman, Kathiresan, Lau, Doreen, Saju, Jolly M., Musthaq SK, Syed, Lunny, Declan Patrick, Vij, Shubha, Orbán, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012279/
https://www.ncbi.nlm.nih.gov/pubmed/27635341
http://dx.doi.org/10.7717/peerj.2377
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author Purushothaman, Kathiresan
Lau, Doreen
Saju, Jolly M.
Musthaq SK, Syed
Lunny, Declan Patrick
Vij, Shubha
Orbán, László
author_facet Purushothaman, Kathiresan
Lau, Doreen
Saju, Jolly M.
Musthaq SK, Syed
Lunny, Declan Patrick
Vij, Shubha
Orbán, László
author_sort Purushothaman, Kathiresan
collection PubMed
description Asian seabass (Lates calcarifer) is a food fish of increasing aquaculture importance. In order to improve our understanding on the digestive system and feeding of this species, morphological and histological features of the gut were studied. Morphologically, the Asian seabass gut is defined by a short and muscular esophagus, well-developed stomach and comparatively short intestine. Mucous secreting goblet cells reactive to PAS (Periodic Acid Schiff) and AB (Alcian Blue) stain were present throughout the esophagus. The stomach was sac-like and could be distinguished into the cardiac, fundic and pyloric regions. Gastric glands and mucus cells were predominately present in the cardiac and fundic regions. Five finger-like pyloric caeca were present between the stomach and intestine. The intestine was a short, tubular structure with no morphological differences between the various regions. Histologically, the intestinal regions were similar, the main difference being in the number of goblet cells that increased from anterior to posterior intestine, with 114 ± 9, 153 ± 7 and 317 ± 21 goblet cells in the anterior, mid and posterior regions, respectively. The intestinal epithelium stained positively for PAS, but the staining was stronger for acidic glycoproteins. The rectum was similar to intestine, except for increased goblet cell numbers (anterior rectum: 529 ± 26; posterior rectum: 745 ± 29). Gut morpho-histology did not respond to salinity changes, however, there was a significant reduction of mucosal height, goblet cell numbers and muscularis thickness upon food deprivation.
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spelling pubmed-50122792016-09-15 Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer) Purushothaman, Kathiresan Lau, Doreen Saju, Jolly M. Musthaq SK, Syed Lunny, Declan Patrick Vij, Shubha Orbán, László PeerJ Aquaculture, Fisheries and Fish Science Asian seabass (Lates calcarifer) is a food fish of increasing aquaculture importance. In order to improve our understanding on the digestive system and feeding of this species, morphological and histological features of the gut were studied. Morphologically, the Asian seabass gut is defined by a short and muscular esophagus, well-developed stomach and comparatively short intestine. Mucous secreting goblet cells reactive to PAS (Periodic Acid Schiff) and AB (Alcian Blue) stain were present throughout the esophagus. The stomach was sac-like and could be distinguished into the cardiac, fundic and pyloric regions. Gastric glands and mucus cells were predominately present in the cardiac and fundic regions. Five finger-like pyloric caeca were present between the stomach and intestine. The intestine was a short, tubular structure with no morphological differences between the various regions. Histologically, the intestinal regions were similar, the main difference being in the number of goblet cells that increased from anterior to posterior intestine, with 114 ± 9, 153 ± 7 and 317 ± 21 goblet cells in the anterior, mid and posterior regions, respectively. The intestinal epithelium stained positively for PAS, but the staining was stronger for acidic glycoproteins. The rectum was similar to intestine, except for increased goblet cell numbers (anterior rectum: 529 ± 26; posterior rectum: 745 ± 29). Gut morpho-histology did not respond to salinity changes, however, there was a significant reduction of mucosal height, goblet cell numbers and muscularis thickness upon food deprivation. PeerJ Inc. 2016-08-24 /pmc/articles/PMC5012279/ /pubmed/27635341 http://dx.doi.org/10.7717/peerj.2377 Text en ©2016 Purushothaman et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Purushothaman, Kathiresan
Lau, Doreen
Saju, Jolly M.
Musthaq SK, Syed
Lunny, Declan Patrick
Vij, Shubha
Orbán, László
Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title_full Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title_fullStr Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title_full_unstemmed Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title_short Morpho-histological characterisation of the alimentary canal of an important food fish, Asian seabass (Lates calcarifer)
title_sort morpho-histological characterisation of the alimentary canal of an important food fish, asian seabass (lates calcarifer)
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012279/
https://www.ncbi.nlm.nih.gov/pubmed/27635341
http://dx.doi.org/10.7717/peerj.2377
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