Cargando…

A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy

With high productivity and stress tolerance, numerous grass genera of the Andropogoneae have emerged as candidates for bioenergy production. To optimize these candidates, research examining the genetic architecture of yield, carbon partitioning, and composition is required to advance breeding object...

Descripción completa

Detalles Bibliográficos
Autores principales: Brenton, Zachary W., Cooper, Elizabeth A., Myers, Mathew T., Boyles, Richard E., Shakoor, Nadia, Zielinski, Kelsey J., Rauh, Bradley L., Bridges, William C., Morris, Geoffrey P., Kresovich, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012387/
https://www.ncbi.nlm.nih.gov/pubmed/27356613
http://dx.doi.org/10.1534/genetics.115.183947
_version_ 1782451996022800384
author Brenton, Zachary W.
Cooper, Elizabeth A.
Myers, Mathew T.
Boyles, Richard E.
Shakoor, Nadia
Zielinski, Kelsey J.
Rauh, Bradley L.
Bridges, William C.
Morris, Geoffrey P.
Kresovich, Stephen
author_facet Brenton, Zachary W.
Cooper, Elizabeth A.
Myers, Mathew T.
Boyles, Richard E.
Shakoor, Nadia
Zielinski, Kelsey J.
Rauh, Bradley L.
Bridges, William C.
Morris, Geoffrey P.
Kresovich, Stephen
author_sort Brenton, Zachary W.
collection PubMed
description With high productivity and stress tolerance, numerous grass genera of the Andropogoneae have emerged as candidates for bioenergy production. To optimize these candidates, research examining the genetic architecture of yield, carbon partitioning, and composition is required to advance breeding objectives. Significant progress has been made developing genetic and genomic resources for Andropogoneae, and advances in comparative and computational genomics have enabled research examining the genetic basis of photosynthesis, carbon partitioning, composition, and sink strength. To provide a pivotal resource aimed at developing a comparative understanding of key bioenergy traits in the Andropogoneae, we have established and characterized an association panel of 390 racially, geographically, and phenotypically diverse Sorghum bicolor accessions with 232,303 genetic markers. Sorghum bicolor was selected because of its genomic simplicity, phenotypic diversity, significant genomic tools, and its agricultural productivity and resilience. We have demonstrated the value of sorghum as a functional model for candidate gene discovery for bioenergy Andropogoneae by performing genome-wide association analysis for two contrasting phenotypes representing key components of structural and non-structural carbohydrates. We identified potential genes, including a cellulase enzyme and a vacuolar transporter, associated with increased non-structural carbohydrates that could lead to bioenergy sorghum improvement. Although our analysis identified genes with potentially clear functions, other candidates did not have assigned functions, suggesting novel molecular mechanisms for carbon partitioning traits. These results, combined with our characterization of phenotypic and genetic diversity and the public accessibility of each accession and genomic data, demonstrate the value of this resource and provide a foundation for future improvement of sorghum and related grasses for bioenergy production.
format Online
Article
Text
id pubmed-5012387
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Genetics Society of America
record_format MEDLINE/PubMed
spelling pubmed-50123872016-09-07 A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy Brenton, Zachary W. Cooper, Elizabeth A. Myers, Mathew T. Boyles, Richard E. Shakoor, Nadia Zielinski, Kelsey J. Rauh, Bradley L. Bridges, William C. Morris, Geoffrey P. Kresovich, Stephen Genetics Investigations With high productivity and stress tolerance, numerous grass genera of the Andropogoneae have emerged as candidates for bioenergy production. To optimize these candidates, research examining the genetic architecture of yield, carbon partitioning, and composition is required to advance breeding objectives. Significant progress has been made developing genetic and genomic resources for Andropogoneae, and advances in comparative and computational genomics have enabled research examining the genetic basis of photosynthesis, carbon partitioning, composition, and sink strength. To provide a pivotal resource aimed at developing a comparative understanding of key bioenergy traits in the Andropogoneae, we have established and characterized an association panel of 390 racially, geographically, and phenotypically diverse Sorghum bicolor accessions with 232,303 genetic markers. Sorghum bicolor was selected because of its genomic simplicity, phenotypic diversity, significant genomic tools, and its agricultural productivity and resilience. We have demonstrated the value of sorghum as a functional model for candidate gene discovery for bioenergy Andropogoneae by performing genome-wide association analysis for two contrasting phenotypes representing key components of structural and non-structural carbohydrates. We identified potential genes, including a cellulase enzyme and a vacuolar transporter, associated with increased non-structural carbohydrates that could lead to bioenergy sorghum improvement. Although our analysis identified genes with potentially clear functions, other candidates did not have assigned functions, suggesting novel molecular mechanisms for carbon partitioning traits. These results, combined with our characterization of phenotypic and genetic diversity and the public accessibility of each accession and genomic data, demonstrate the value of this resource and provide a foundation for future improvement of sorghum and related grasses for bioenergy production. Genetics Society of America 2016-09 2016-06-27 /pmc/articles/PMC5012387/ /pubmed/27356613 http://dx.doi.org/10.1534/genetics.115.183947 Text en Copyright © 2016 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle Investigations
Brenton, Zachary W.
Cooper, Elizabeth A.
Myers, Mathew T.
Boyles, Richard E.
Shakoor, Nadia
Zielinski, Kelsey J.
Rauh, Bradley L.
Bridges, William C.
Morris, Geoffrey P.
Kresovich, Stephen
A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title_full A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title_fullStr A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title_full_unstemmed A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title_short A Genomic Resource for the Development, Improvement, and Exploitation of Sorghum for Bioenergy
title_sort genomic resource for the development, improvement, and exploitation of sorghum for bioenergy
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012387/
https://www.ncbi.nlm.nih.gov/pubmed/27356613
http://dx.doi.org/10.1534/genetics.115.183947
work_keys_str_mv AT brentonzacharyw agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT cooperelizabetha agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT myersmathewt agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT boylesricharde agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT shakoornadia agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT zielinskikelseyj agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT rauhbradleyl agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT bridgeswilliamc agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT morrisgeoffreyp agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT kresovichstephen agenomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT brentonzacharyw genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT cooperelizabetha genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT myersmathewt genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT boylesricharde genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT shakoornadia genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT zielinskikelseyj genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT rauhbradleyl genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT bridgeswilliamc genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT morrisgeoffreyp genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy
AT kresovichstephen genomicresourceforthedevelopmentimprovementandexploitationofsorghumforbioenergy