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Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model

Musculoskeletal modelling has become a valuable tool with which to understand how neural, muscular, skeletal and other tissues are integrated to produce movement. Most musculoskeletal modelling work has to date focused on humans or their close relatives, with few examples of quadrupedal animal limb...

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Autores principales: Charles, James P., Cappellari, Ornella, Spence, Andrew J., Wells, Dominic J., Hutchinson, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013061/
https://www.ncbi.nlm.nih.gov/pubmed/27173448
http://dx.doi.org/10.1111/joa.12461
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author Charles, James P.
Cappellari, Ornella
Spence, Andrew J.
Wells, Dominic J.
Hutchinson, John R.
author_facet Charles, James P.
Cappellari, Ornella
Spence, Andrew J.
Wells, Dominic J.
Hutchinson, John R.
author_sort Charles, James P.
collection PubMed
description Musculoskeletal modelling has become a valuable tool with which to understand how neural, muscular, skeletal and other tissues are integrated to produce movement. Most musculoskeletal modelling work has to date focused on humans or their close relatives, with few examples of quadrupedal animal limb models. A musculoskeletal model of the mouse hindlimb could have broad utility for questions in medicine, genetics, locomotion and neuroscience. This is due to this species’ position as a premier model of human disease, having an array of genetic tools for manipulation of the animal in vivo, and being a small quadruped, a category for which few models exist. Here, the methods used to develop the first three‐dimensional (3D) model of a mouse hindlimb and pelvis are described. The model, which represents bones, joints and 39 musculotendon units, was created through a combination of previously gathered muscle architecture data from microdissections, contrast‐enhanced micro‐computed tomography (CT) scanning and digital segmentation. The model allowed muscle moment arms as well as muscle forces to be estimated for each musculotendon unit throughout a range of joint rotations. Moment arm analysis supported the reliability of musculotendon unit placement within the model, and comparison to a previously published rat hindlimb model further supported the model's reliability. A sensitivity analysis performed on both the force‐generating parameters and muscle's attachment points of the model indicated that the maximal isometric muscle moment is generally most sensitive to changes in either tendon slack length or the coordinates of insertion, although the degree to which the moment is affected depends on several factors. This model represents the first step in the creation of a fully dynamic 3D computer model of the mouse hindlimb and pelvis that has application to neuromuscular disease, comparative biomechanics and the neuromechanical basis of movement. Capturing the morphology and dynamics of the limb, it enables future dissection of the complex interactions between the nervous and musculoskeletal systems as well as the environment.
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spelling pubmed-50130612016-10-06 Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model Charles, James P. Cappellari, Ornella Spence, Andrew J. Wells, Dominic J. Hutchinson, John R. J Anat Original Articles Musculoskeletal modelling has become a valuable tool with which to understand how neural, muscular, skeletal and other tissues are integrated to produce movement. Most musculoskeletal modelling work has to date focused on humans or their close relatives, with few examples of quadrupedal animal limb models. A musculoskeletal model of the mouse hindlimb could have broad utility for questions in medicine, genetics, locomotion and neuroscience. This is due to this species’ position as a premier model of human disease, having an array of genetic tools for manipulation of the animal in vivo, and being a small quadruped, a category for which few models exist. Here, the methods used to develop the first three‐dimensional (3D) model of a mouse hindlimb and pelvis are described. The model, which represents bones, joints and 39 musculotendon units, was created through a combination of previously gathered muscle architecture data from microdissections, contrast‐enhanced micro‐computed tomography (CT) scanning and digital segmentation. The model allowed muscle moment arms as well as muscle forces to be estimated for each musculotendon unit throughout a range of joint rotations. Moment arm analysis supported the reliability of musculotendon unit placement within the model, and comparison to a previously published rat hindlimb model further supported the model's reliability. A sensitivity analysis performed on both the force‐generating parameters and muscle's attachment points of the model indicated that the maximal isometric muscle moment is generally most sensitive to changes in either tendon slack length or the coordinates of insertion, although the degree to which the moment is affected depends on several factors. This model represents the first step in the creation of a fully dynamic 3D computer model of the mouse hindlimb and pelvis that has application to neuromuscular disease, comparative biomechanics and the neuromechanical basis of movement. Capturing the morphology and dynamics of the limb, it enables future dissection of the complex interactions between the nervous and musculoskeletal systems as well as the environment. John Wiley and Sons Inc. 2016-05-12 2016-10 /pmc/articles/PMC5013061/ /pubmed/27173448 http://dx.doi.org/10.1111/joa.12461 Text en © 2016 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Charles, James P.
Cappellari, Ornella
Spence, Andrew J.
Wells, Dominic J.
Hutchinson, John R.
Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title_full Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title_fullStr Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title_full_unstemmed Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title_short Muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
title_sort muscle moment arms and sensitivity analysis of a mouse hindlimb musculoskeletal model
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013061/
https://www.ncbi.nlm.nih.gov/pubmed/27173448
http://dx.doi.org/10.1111/joa.12461
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