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FtsZ-Dependent Elongation of a Coccoid Bacterium
A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morpholo...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013293/ https://www.ncbi.nlm.nih.gov/pubmed/27601570 http://dx.doi.org/10.1128/mBio.00908-16 |
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author | Pereira, Ana R. Hsin, Jen Król, Ewa Tavares, Andreia C. Flores, Pierre Hoiczyk, Egbert Ng, Natalie Dajkovic, Alex Brun, Yves V. VanNieuwenhze, Michael S. Roemer, Terry Carballido-Lopez, Rut Scheffers, Dirk-Jan Huang, Kerwyn Casey Pinho, Mariana G. |
author_facet | Pereira, Ana R. Hsin, Jen Król, Ewa Tavares, Andreia C. Flores, Pierre Hoiczyk, Egbert Ng, Natalie Dajkovic, Alex Brun, Yves V. VanNieuwenhze, Michael S. Roemer, Terry Carballido-Lopez, Rut Scheffers, Dirk-Jan Huang, Kerwyn Casey Pinho, Mariana G. |
author_sort | Pereira, Ana R. |
collection | PubMed |
description | A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morphology, as a transition from a spherical to a rod-like shape has never been observed in bacteria. Here we show that a Staphylococcus aureus mutant (M5) expressing the ftsZ(G193D) allele exhibits elongated cells. Molecular dynamics simulations and in vitro studies indicate that FtsZ(G193D) filaments are more twisted and shorter than wild-type filaments. In vivo, M5 cell wall deposition is initiated asymmetrically, only on one side of the cell, and progresses into a helical pattern rather than into a constricting ring as in wild-type cells. This helical pattern of wall insertion leads to elongation, as in rod-shaped cells. Thus, structural flexibility of FtsZ filaments can result in an FtsZ-dependent mechanism for generating elongated cells from cocci. |
format | Online Article Text |
id | pubmed-5013293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-50132932016-09-16 FtsZ-Dependent Elongation of a Coccoid Bacterium Pereira, Ana R. Hsin, Jen Król, Ewa Tavares, Andreia C. Flores, Pierre Hoiczyk, Egbert Ng, Natalie Dajkovic, Alex Brun, Yves V. VanNieuwenhze, Michael S. Roemer, Terry Carballido-Lopez, Rut Scheffers, Dirk-Jan Huang, Kerwyn Casey Pinho, Mariana G. mBio Research Article A mechanistic understanding of the determination and maintenance of the simplest bacterial cell shape, a sphere, remains elusive compared with that of more complex shapes. Cocci seem to lack a dedicated elongation machinery, and a spherical shape has been considered an evolutionary dead-end morphology, as a transition from a spherical to a rod-like shape has never been observed in bacteria. Here we show that a Staphylococcus aureus mutant (M5) expressing the ftsZ(G193D) allele exhibits elongated cells. Molecular dynamics simulations and in vitro studies indicate that FtsZ(G193D) filaments are more twisted and shorter than wild-type filaments. In vivo, M5 cell wall deposition is initiated asymmetrically, only on one side of the cell, and progresses into a helical pattern rather than into a constricting ring as in wild-type cells. This helical pattern of wall insertion leads to elongation, as in rod-shaped cells. Thus, structural flexibility of FtsZ filaments can result in an FtsZ-dependent mechanism for generating elongated cells from cocci. American Society for Microbiology 2016-09-06 /pmc/articles/PMC5013293/ /pubmed/27601570 http://dx.doi.org/10.1128/mBio.00908-16 Text en Copyright © 2016 Pereira et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Pereira, Ana R. Hsin, Jen Król, Ewa Tavares, Andreia C. Flores, Pierre Hoiczyk, Egbert Ng, Natalie Dajkovic, Alex Brun, Yves V. VanNieuwenhze, Michael S. Roemer, Terry Carballido-Lopez, Rut Scheffers, Dirk-Jan Huang, Kerwyn Casey Pinho, Mariana G. FtsZ-Dependent Elongation of a Coccoid Bacterium |
title | FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_full | FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_fullStr | FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_full_unstemmed | FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_short | FtsZ-Dependent Elongation of a Coccoid Bacterium |
title_sort | ftsz-dependent elongation of a coccoid bacterium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013293/ https://www.ncbi.nlm.nih.gov/pubmed/27601570 http://dx.doi.org/10.1128/mBio.00908-16 |
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