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EIGER detector: application in macromolecular crystallography

The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz a...

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Autores principales: Casanas, Arnau, Warshamanage, Rangana, Finke, Aaron D., Panepucci, Ezequiel, Olieric, Vincent, Nöll, Anne, Tampé, Robert, Brandstetter, Stefan, Förster, Andreas, Mueller, Marcus, Schulze-Briese, Clemens, Bunk, Oliver, Wang, Meitian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013597/
https://www.ncbi.nlm.nih.gov/pubmed/27599736
http://dx.doi.org/10.1107/S2059798316012304
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author Casanas, Arnau
Warshamanage, Rangana
Finke, Aaron D.
Panepucci, Ezequiel
Olieric, Vincent
Nöll, Anne
Tampé, Robert
Brandstetter, Stefan
Förster, Andreas
Mueller, Marcus
Schulze-Briese, Clemens
Bunk, Oliver
Wang, Meitian
author_facet Casanas, Arnau
Warshamanage, Rangana
Finke, Aaron D.
Panepucci, Ezequiel
Olieric, Vincent
Nöll, Anne
Tampé, Robert
Brandstetter, Stefan
Förster, Andreas
Mueller, Marcus
Schulze-Briese, Clemens
Bunk, Oliver
Wang, Meitian
author_sort Casanas, Arnau
collection PubMed
description The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine φ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.
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spelling pubmed-50135972016-09-20 EIGER detector: application in macromolecular crystallography Casanas, Arnau Warshamanage, Rangana Finke, Aaron D. Panepucci, Ezequiel Olieric, Vincent Nöll, Anne Tampé, Robert Brandstetter, Stefan Förster, Andreas Mueller, Marcus Schulze-Briese, Clemens Bunk, Oliver Wang, Meitian Acta Crystallogr D Struct Biol Research Papers The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine φ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed. International Union of Crystallography 2016-08-31 /pmc/articles/PMC5013597/ /pubmed/27599736 http://dx.doi.org/10.1107/S2059798316012304 Text en © Casanas et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Casanas, Arnau
Warshamanage, Rangana
Finke, Aaron D.
Panepucci, Ezequiel
Olieric, Vincent
Nöll, Anne
Tampé, Robert
Brandstetter, Stefan
Förster, Andreas
Mueller, Marcus
Schulze-Briese, Clemens
Bunk, Oliver
Wang, Meitian
EIGER detector: application in macromolecular crystallography
title EIGER detector: application in macromolecular crystallography
title_full EIGER detector: application in macromolecular crystallography
title_fullStr EIGER detector: application in macromolecular crystallography
title_full_unstemmed EIGER detector: application in macromolecular crystallography
title_short EIGER detector: application in macromolecular crystallography
title_sort eiger detector: application in macromolecular crystallography
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013597/
https://www.ncbi.nlm.nih.gov/pubmed/27599736
http://dx.doi.org/10.1107/S2059798316012304
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