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Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

MHC tetramers are an essential tool for characterizing antigen-specific CD4+ T cells. However, their ex vivo analysis is limited by the large sample requirements. Here we demonstrate a combinatorial staining approach that allows simultaneous characterization of multiple specificities to address this...

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Detalles Bibliográficos
Autores principales:	Uchtenhagen, Hannes, Rims, Cliff, Blahnik, Gabriele, Chow, I-Ting, Kwok, William W., Buckner, Jane H., James, Eddie A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013714/ https://www.ncbi.nlm.nih.gov/pubmed/27571776 http://dx.doi.org/10.1038/ncomms12614

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