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pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences

Increasing demand for large-scale synthesis of in vitro transcribed (IVT) mRNA is being driven by the increasing use of mRNA for transient gene expression in cell engineering and therapeutic applications. An important determinant of IVT mRNA potency is the 3′ polyadenosine (poly(A)) tail, the length...

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Detalles Bibliográficos
Autores principales: Grier, Alexandra E, Burleigh, Stephen, Sahni, Jaya, Clough, Courtnee A, Cardot, Victoire, Choe, Dongwook C, Krutein, Michelle C, Rawlings, David J, Jensen, Michael C, Scharenberg, Andrew M, Jacoby, Kyle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5014522/
https://www.ncbi.nlm.nih.gov/pubmed/27093168
http://dx.doi.org/10.1038/mtna.2016.21