CRISPR guide RNA design for research applications

The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs (gRNAs). Here, we review the state of the art in CRISPR gRNA design for researc...

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Autores principales: Mohr, Stephanie E., Hu, Yanhui, Ewen‐Campen, Benjamin, Housden, Benjamin E., Viswanatha, Raghuvir, Perrimon, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Special Issue: CRISPR/Cas9 Gene Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5014588/
https://www.ncbi.nlm.nih.gov/pubmed/27276584
http://dx.doi.org/10.1111/febs.13777
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author Mohr, Stephanie E.
Hu, Yanhui
Ewen‐Campen, Benjamin
Housden, Benjamin E.
Viswanatha, Raghuvir
Perrimon, Norbert
author_facet Mohr, Stephanie E.
Hu, Yanhui
Ewen‐Campen, Benjamin
Housden, Benjamin E.
Viswanatha, Raghuvir
Perrimon, Norbert
author_sort Mohr, Stephanie E.
collection PubMed
description The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs (gRNAs). Here, we review the state of the art in CRISPR gRNA design for research applications of the CRISPR‐Cas9 system, including knockout, activation, and inhibition. Notably, achieving good gRNA design is not solely dependent on innovations in CRISPR technology. Good design and design tools also rely on availability of high‐quality genome sequence and gene annotations, as well as on availability of accumulated data regarding off‐targets and effectiveness metrics.
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spelling pubmed-50145882016-10-19 CRISPR guide RNA design for research applications Mohr, Stephanie E. Hu, Yanhui Ewen‐Campen, Benjamin Housden, Benjamin E. Viswanatha, Raghuvir Perrimon, Norbert FEBS J Special Issue: CRISPR/Cas9 Gene Editing The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs (gRNAs). Here, we review the state of the art in CRISPR gRNA design for research applications of the CRISPR‐Cas9 system, including knockout, activation, and inhibition. Notably, achieving good gRNA design is not solely dependent on innovations in CRISPR technology. Good design and design tools also rely on availability of high‐quality genome sequence and gene annotations, as well as on availability of accumulated data regarding off‐targets and effectiveness metrics. John Wiley and Sons Inc. 2016-06-22 2016-09 /pmc/articles/PMC5014588/ /pubmed/27276584 http://dx.doi.org/10.1111/febs.13777 Text en © 2016 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Special Issue: CRISPR/Cas9 Gene Editing
Mohr, Stephanie E.
Hu, Yanhui
Ewen‐Campen, Benjamin
Housden, Benjamin E.
Viswanatha, Raghuvir
Perrimon, Norbert
CRISPR guide RNA design for research applications
title CRISPR guide RNA design for research applications
title_full CRISPR guide RNA design for research applications
title_fullStr CRISPR guide RNA design for research applications
title_full_unstemmed CRISPR guide RNA design for research applications
title_short CRISPR guide RNA design for research applications
title_sort crispr guide rna design for research applications
topic Special Issue: CRISPR/Cas9 Gene Editing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5014588/
https://www.ncbi.nlm.nih.gov/pubmed/27276584
http://dx.doi.org/10.1111/febs.13777
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AT housdenbenjamine crisprguidernadesignforresearchapplications
AT viswanatharaghuvir crisprguidernadesignforresearchapplications
AT perrimonnorbert crisprguidernadesignforresearchapplications

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