Cargando…
Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia fo...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015030/ https://www.ncbi.nlm.nih.gov/pubmed/27642493 http://dx.doi.org/10.1155/2016/3406802 |
_version_ | 1782452368889085952 |
---|---|
author | Yao, Chen Li, Gang Qian, Yeyong Cai, Ming Yin, Hong Xiao, Li Tang, Wei Guo, Fengjie Shi, Bingyi |
author_facet | Yao, Chen Li, Gang Qian, Yeyong Cai, Ming Yin, Hong Xiao, Li Tang, Wei Guo, Fengjie Shi, Bingyi |
author_sort | Yao, Chen |
collection | PubMed |
description | To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX. |
format | Online Article Text |
id | pubmed-5015030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50150302016-09-18 Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia Yao, Chen Li, Gang Qian, Yeyong Cai, Ming Yin, Hong Xiao, Li Tang, Wei Guo, Fengjie Shi, Bingyi Oxid Med Cell Longev Research Article To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX. Hindawi Publishing Corporation 2016 2016-08-25 /pmc/articles/PMC5015030/ /pubmed/27642493 http://dx.doi.org/10.1155/2016/3406802 Text en Copyright © 2016 Chen Yao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yao, Chen Li, Gang Qian, Yeyong Cai, Ming Yin, Hong Xiao, Li Tang, Wei Guo, Fengjie Shi, Bingyi Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title | Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title_full | Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title_fullStr | Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title_full_unstemmed | Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title_short | Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia |
title_sort | protection of pentoxifylline against testis injury induced by intermittent hypobaric hypoxia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015030/ https://www.ncbi.nlm.nih.gov/pubmed/27642493 http://dx.doi.org/10.1155/2016/3406802 |
work_keys_str_mv | AT yaochen protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT ligang protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT qianyeyong protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT caiming protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT yinhong protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT xiaoli protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT tangwei protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT guofengjie protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia AT shibingyi protectionofpentoxifyllineagainsttestisinjuryinducedbyintermittenthypobarichypoxia |