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Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia

To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia fo...

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Autores principales: Yao, Chen, Li, Gang, Qian, Yeyong, Cai, Ming, Yin, Hong, Xiao, Li, Tang, Wei, Guo, Fengjie, Shi, Bingyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015030/
https://www.ncbi.nlm.nih.gov/pubmed/27642493
http://dx.doi.org/10.1155/2016/3406802
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author Yao, Chen
Li, Gang
Qian, Yeyong
Cai, Ming
Yin, Hong
Xiao, Li
Tang, Wei
Guo, Fengjie
Shi, Bingyi
author_facet Yao, Chen
Li, Gang
Qian, Yeyong
Cai, Ming
Yin, Hong
Xiao, Li
Tang, Wei
Guo, Fengjie
Shi, Bingyi
author_sort Yao, Chen
collection PubMed
description To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX.
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spelling pubmed-50150302016-09-18 Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia Yao, Chen Li, Gang Qian, Yeyong Cai, Ming Yin, Hong Xiao, Li Tang, Wei Guo, Fengjie Shi, Bingyi Oxid Med Cell Longev Research Article To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days. PTX was administered for 32 days. Blood and tissue samples were collected 7 days thereafter. Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples. Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen's testicular scoring system. Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry. Sperm count, motility, and density were assessed to determine epididymal sperm quality. IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression. Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells. Oral administration of PTX improved the pathological changes in the testis. IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX. Hindawi Publishing Corporation 2016 2016-08-25 /pmc/articles/PMC5015030/ /pubmed/27642493 http://dx.doi.org/10.1155/2016/3406802 Text en Copyright © 2016 Chen Yao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yao, Chen
Li, Gang
Qian, Yeyong
Cai, Ming
Yin, Hong
Xiao, Li
Tang, Wei
Guo, Fengjie
Shi, Bingyi
Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title_full Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title_fullStr Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title_full_unstemmed Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title_short Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
title_sort protection of pentoxifylline against testis injury induced by intermittent hypobaric hypoxia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015030/
https://www.ncbi.nlm.nih.gov/pubmed/27642493
http://dx.doi.org/10.1155/2016/3406802
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