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Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis

Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium P...

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Autores principales: Chen, Wen-Jen, Kuo, Tzu-Yen, Hsieh, Feng-Chia, Chen, Pi-Yu, Wang, Chang-Sheng, Shih, Yu-Ling, Lai, Ying-Mi, Liu, Je-Ruei, Yang, Yu-Liang, Shih, Ming-Che
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015096/
https://www.ncbi.nlm.nih.gov/pubmed/27605490
http://dx.doi.org/10.1038/srep32950
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author Chen, Wen-Jen
Kuo, Tzu-Yen
Hsieh, Feng-Chia
Chen, Pi-Yu
Wang, Chang-Sheng
Shih, Yu-Ling
Lai, Ying-Mi
Liu, Je-Ruei
Yang, Yu-Liang
Shih, Ming-Che
author_facet Chen, Wen-Jen
Kuo, Tzu-Yen
Hsieh, Feng-Chia
Chen, Pi-Yu
Wang, Chang-Sheng
Shih, Yu-Ling
Lai, Ying-Mi
Liu, Je-Ruei
Yang, Yu-Liang
Shih, Ming-Che
author_sort Chen, Wen-Jen
collection PubMed
description Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity against Xoo. Using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secreted by P. taiwanensis that could inhibit the growth of Xoo. Through Tn5 mutagenesis of P. taiwanensis, we showed that mutations in genes that encode components of the type VI secretion system (T6SS) as well as biosynthesis and maturation of pyoverdine resulted in reduced toxicity against Xoo. Our results indicated that T6SS is involved in the secretion of endogenous pyoverdine. Mutations in T6SS component genes affected the secretion of mature pyoverdine from the periplasmic space into the extracellular medium after pyoverdine precursor is transferred to the periplasm by the inner membrane transporter PvdE. In addition, we also showed that other export systems, i.e., the PvdRT-OpmQ and MexAB-OprM efflux systems (for which there have been previous suggestions of involvement) and the type II secretion system (T2SS), are not involved in pyoverdine secretion.
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spelling pubmed-50150962016-09-12 Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis Chen, Wen-Jen Kuo, Tzu-Yen Hsieh, Feng-Chia Chen, Pi-Yu Wang, Chang-Sheng Shih, Yu-Ling Lai, Ying-Mi Liu, Je-Ruei Yang, Yu-Liang Shih, Ming-Che Sci Rep Article Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity against Xoo. Using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secreted by P. taiwanensis that could inhibit the growth of Xoo. Through Tn5 mutagenesis of P. taiwanensis, we showed that mutations in genes that encode components of the type VI secretion system (T6SS) as well as biosynthesis and maturation of pyoverdine resulted in reduced toxicity against Xoo. Our results indicated that T6SS is involved in the secretion of endogenous pyoverdine. Mutations in T6SS component genes affected the secretion of mature pyoverdine from the periplasmic space into the extracellular medium after pyoverdine precursor is transferred to the periplasm by the inner membrane transporter PvdE. In addition, we also showed that other export systems, i.e., the PvdRT-OpmQ and MexAB-OprM efflux systems (for which there have been previous suggestions of involvement) and the type II secretion system (T2SS), are not involved in pyoverdine secretion. Nature Publishing Group 2016-09-08 /pmc/articles/PMC5015096/ /pubmed/27605490 http://dx.doi.org/10.1038/srep32950 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Wen-Jen
Kuo, Tzu-Yen
Hsieh, Feng-Chia
Chen, Pi-Yu
Wang, Chang-Sheng
Shih, Yu-Ling
Lai, Ying-Mi
Liu, Je-Ruei
Yang, Yu-Liang
Shih, Ming-Che
Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title_full Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title_fullStr Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title_full_unstemmed Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title_short Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis
title_sort involvement of type vi secretion system in secretion of iron chelator pyoverdine in pseudomonas taiwanensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015096/
https://www.ncbi.nlm.nih.gov/pubmed/27605490
http://dx.doi.org/10.1038/srep32950
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