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Complete genome sequence of Halomonas sp. R5-57

The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance...

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Autores principales: Williamson, Adele, De Santi, Concetta, Altermark, Bjørn, Karlsen, Christian, Hjerde, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015195/
https://www.ncbi.nlm.nih.gov/pubmed/27610212
http://dx.doi.org/10.1186/s40793-016-0192-4
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author Williamson, Adele
De Santi, Concetta
Altermark, Bjørn
Karlsen, Christian
Hjerde, Erik
author_facet Williamson, Adele
De Santi, Concetta
Altermark, Bjørn
Karlsen, Christian
Hjerde, Erik
author_sort Williamson, Adele
collection PubMed
description The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance over a wide range of temperatures and has extra-cellular hydrolytic activities with several substrates, indicating it secretes enzymes which may function in high salinity conditions. Genome sequencing identified the genes involved in the biosynthesis of the osmoprotectant ectoine, which has applications in food processing and pharmacy, as well as those involved in production of polyhydroxyalkanoates, which can serve as precursors to bioplastics. The percentage identity of these biosynthetic genes from Halomonas sp. R5-57 and current production strains varies between 99 % for some to 69 % for others, thus it is plausible that R5-57 may have a different production capacity to currently used strains, or that in the case of PHAs, the properties of the final product may vary. Here we present the finished genome sequence (LN813019) of Halomonas sp. R5-57 which will facilitate exploitation of this bacterium; either as a whole-cell production host, or by recombinant expression of its individual enzymes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-016-0192-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-50151952016-09-09 Complete genome sequence of Halomonas sp. R5-57 Williamson, Adele De Santi, Concetta Altermark, Bjørn Karlsen, Christian Hjerde, Erik Stand Genomic Sci Extended Genome Report The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance over a wide range of temperatures and has extra-cellular hydrolytic activities with several substrates, indicating it secretes enzymes which may function in high salinity conditions. Genome sequencing identified the genes involved in the biosynthesis of the osmoprotectant ectoine, which has applications in food processing and pharmacy, as well as those involved in production of polyhydroxyalkanoates, which can serve as precursors to bioplastics. The percentage identity of these biosynthetic genes from Halomonas sp. R5-57 and current production strains varies between 99 % for some to 69 % for others, thus it is plausible that R5-57 may have a different production capacity to currently used strains, or that in the case of PHAs, the properties of the final product may vary. Here we present the finished genome sequence (LN813019) of Halomonas sp. R5-57 which will facilitate exploitation of this bacterium; either as a whole-cell production host, or by recombinant expression of its individual enzymes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-016-0192-4) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-07 /pmc/articles/PMC5015195/ /pubmed/27610212 http://dx.doi.org/10.1186/s40793-016-0192-4 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Extended Genome Report
Williamson, Adele
De Santi, Concetta
Altermark, Bjørn
Karlsen, Christian
Hjerde, Erik
Complete genome sequence of Halomonas sp. R5-57
title Complete genome sequence of Halomonas sp. R5-57
title_full Complete genome sequence of Halomonas sp. R5-57
title_fullStr Complete genome sequence of Halomonas sp. R5-57
title_full_unstemmed Complete genome sequence of Halomonas sp. R5-57
title_short Complete genome sequence of Halomonas sp. R5-57
title_sort complete genome sequence of halomonas sp. r5-57
topic Extended Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015195/
https://www.ncbi.nlm.nih.gov/pubmed/27610212
http://dx.doi.org/10.1186/s40793-016-0192-4
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