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Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria
BACKGROUND: There is an unresolved debate on the best screening method for hematuria as a symptom of glomerulonephritis or urological malignancies. The urinary dipstick is generally considered as an imperfect surrogate for urine microscopy analysis. RESULTS: We designed a study to compare urine micr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015255/ https://www.ncbi.nlm.nih.gov/pubmed/27608628 http://dx.doi.org/10.1186/s13104-016-2240-y |
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author | Bataille, Aurélien Wetzstein, Morgane Hertig, Alexandre Vimont, Sophie Rondeau, Eric Galichon, Pierre |
author_facet | Bataille, Aurélien Wetzstein, Morgane Hertig, Alexandre Vimont, Sophie Rondeau, Eric Galichon, Pierre |
author_sort | Bataille, Aurélien |
collection | PubMed |
description | BACKGROUND: There is an unresolved debate on the best screening method for hematuria as a symptom of glomerulonephritis or urological malignancies. The urinary dipstick is generally considered as an imperfect surrogate for urine microscopy analysis. RESULTS: We designed a study to compare urine microscopy analysis, urinary dipstick and flow cytometry, using controlled dilutions of blood in urine samples from volunteers collected in two different physiologically-relevant conditions (basal state and hyperhydration). We found that although all techniques were 100 % effective in detecting hematuria at basal state, these results were variably reproduced when testing the same final amount of hematuria in urine collected after hyperhydration. Our data shows a variable sensitivity for the detection of hematuria by urine microscopy analysis or flow cytometry, but not by urinary dipstick. CONCLUSIONS: Urinary dipstick qualifies as a better screening test for hematuria than urine microscopy analysis or flow cytometry, as it is sensitive and performs better in unstandardized conditions. It is universally available and also faster and cheaper than cytometric techniques. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-016-2240-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5015255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-50152552016-09-09 Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria Bataille, Aurélien Wetzstein, Morgane Hertig, Alexandre Vimont, Sophie Rondeau, Eric Galichon, Pierre BMC Res Notes Short Report BACKGROUND: There is an unresolved debate on the best screening method for hematuria as a symptom of glomerulonephritis or urological malignancies. The urinary dipstick is generally considered as an imperfect surrogate for urine microscopy analysis. RESULTS: We designed a study to compare urine microscopy analysis, urinary dipstick and flow cytometry, using controlled dilutions of blood in urine samples from volunteers collected in two different physiologically-relevant conditions (basal state and hyperhydration). We found that although all techniques were 100 % effective in detecting hematuria at basal state, these results were variably reproduced when testing the same final amount of hematuria in urine collected after hyperhydration. Our data shows a variable sensitivity for the detection of hematuria by urine microscopy analysis or flow cytometry, but not by urinary dipstick. CONCLUSIONS: Urinary dipstick qualifies as a better screening test for hematuria than urine microscopy analysis or flow cytometry, as it is sensitive and performs better in unstandardized conditions. It is universally available and also faster and cheaper than cytometric techniques. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-016-2240-y) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-08 /pmc/articles/PMC5015255/ /pubmed/27608628 http://dx.doi.org/10.1186/s13104-016-2240-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Bataille, Aurélien Wetzstein, Morgane Hertig, Alexandre Vimont, Sophie Rondeau, Eric Galichon, Pierre Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title | Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title_full | Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title_fullStr | Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title_full_unstemmed | Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title_short | Evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
title_sort | evidence of dipstick superiority over urine microscopy analysis for detection of hematuria |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015255/ https://www.ncbi.nlm.nih.gov/pubmed/27608628 http://dx.doi.org/10.1186/s13104-016-2240-y |
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