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Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus

N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins. Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate struct...

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Autores principales: Li, Mengying, Liu, Xinyu, Liu, Zhixi, Sun, Yi, Liu, Muxing, Wang, Xiaoli, Zhang, Haifeng, Zheng, Xiaobo, Zhang, Zhengguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015852/
https://www.ncbi.nlm.nih.gov/pubmed/27607237
http://dx.doi.org/10.1371/journal.pone.0162243
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author Li, Mengying
Liu, Xinyu
Liu, Zhixi
Sun, Yi
Liu, Muxing
Wang, Xiaoli
Zhang, Haifeng
Zheng, Xiaobo
Zhang, Zhengguang
author_facet Li, Mengying
Liu, Xinyu
Liu, Zhixi
Sun, Yi
Liu, Muxing
Wang, Xiaoli
Zhang, Haifeng
Zheng, Xiaobo
Zhang, Zhengguang
author_sort Li, Mengying
collection PubMed
description N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins. Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures. Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis. Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction. Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth. Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses. Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity. Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
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spelling pubmed-50158522016-09-27 Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus Li, Mengying Liu, Xinyu Liu, Zhixi Sun, Yi Liu, Muxing Wang, Xiaoli Zhang, Haifeng Zheng, Xiaobo Zhang, Zhengguang PLoS One Research Article N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins. Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures. Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis. Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction. Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth. Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses. Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity. Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth. Public Library of Science 2016-09-08 /pmc/articles/PMC5015852/ /pubmed/27607237 http://dx.doi.org/10.1371/journal.pone.0162243 Text en © 2016 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Mengying
Liu, Xinyu
Liu, Zhixi
Sun, Yi
Liu, Muxing
Wang, Xiaoli
Zhang, Haifeng
Zheng, Xiaobo
Zhang, Zhengguang
Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title_full Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title_fullStr Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title_full_unstemmed Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title_short Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus
title_sort glycoside hydrolase mogls2 controls asexual/sexual development, cell wall integrity and infectious growth in the rice blast fungus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5015852/
https://www.ncbi.nlm.nih.gov/pubmed/27607237
http://dx.doi.org/10.1371/journal.pone.0162243
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