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Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury

PURPOSE: We tested the hypothesis that experimental glaucoma (EG) results in greater thinning of the optic nerve head (ONH) neural rim tissue than the peripapillary retinal nerve fiber layer (RNFL) tissue. METHODS: Longitudinal spectral-domain optical coherence tomography (SDOCT) imaging of the ONH...

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Autores principales: Fortune, Brad, Reynaud, Juan, Hardin, Christy, Wang, Lin, Sigal, Ian A., Burgoyne, Claude F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016000/
https://www.ncbi.nlm.nih.gov/pubmed/27564522
http://dx.doi.org/10.1167/iovs.16-20000
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author Fortune, Brad
Reynaud, Juan
Hardin, Christy
Wang, Lin
Sigal, Ian A.
Burgoyne, Claude F.
author_facet Fortune, Brad
Reynaud, Juan
Hardin, Christy
Wang, Lin
Sigal, Ian A.
Burgoyne, Claude F.
author_sort Fortune, Brad
collection PubMed
description PURPOSE: We tested the hypothesis that experimental glaucoma (EG) results in greater thinning of the optic nerve head (ONH) neural rim tissue than the peripapillary retinal nerve fiber layer (RNFL) tissue. METHODS: Longitudinal spectral-domain optical coherence tomography (SDOCT) imaging of the ONH and peripapillary RNFL was performed every other week under manometric IOP control (10 mm Hg) in 51 nonhuman primates (NHP) during baseline and after induction of unilateral EG. The ONH parameter minimum rim area (MRA) was derived from 80 radial B-scans centered on the ONH; RNFL cross-sectional area (RNFLA) from a peripapillary circular B-scan with 12° diameter. RESULTS: In control eyes, MRA was 1.00 ± 0.19 mm(2) at baseline and 1.00 ± 0.19 mm(2) at the final session (P = 0.77), while RNFLA was 0.95 ± 0.09 and 0.95 ± 0.10 mm(2), respectively (P = 0.96). In EG eyes, MRA decreased from 1.00 ± 0.19 mm(2) at baseline to 0.63 ± 0.21 mm(2) at the final session (P < 0.0001), while RNFLA decreased from 0.95 ± 0.09 to 0.74 ± 0.19 mm(2), respectively (P < 0.0001). Thus, MRA decreased by 36.4 ± 20.6% in EG eyes, significantly more than the decrease in RNFLA (21.7 ± 19.4%, P < 0.0001). Other significant changes in EG eyes included increased Bruch's membrane opening (BMO) nonplanarity (P < 0.05), decreased BMO aspect ratio (P < 0.0001), and decreased MRA angle (P < 0.001). Bruch's membrane opening area did not change from baseline in either control or EG eyes (P = 0.27, P = 0.15, respectively). CONCLUSIONS: Optic nerve head neural rim tissue thinning exceeded peripapillary RNFL thinning in NHP EG. These results support the hypothesis that axon bundles are compressed transversely within the ONH rim along with glaucomatous deformation of connective tissues.
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spelling pubmed-50160002016-09-09 Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury Fortune, Brad Reynaud, Juan Hardin, Christy Wang, Lin Sigal, Ian A. Burgoyne, Claude F. Invest Ophthalmol Vis Sci Glaucoma PURPOSE: We tested the hypothesis that experimental glaucoma (EG) results in greater thinning of the optic nerve head (ONH) neural rim tissue than the peripapillary retinal nerve fiber layer (RNFL) tissue. METHODS: Longitudinal spectral-domain optical coherence tomography (SDOCT) imaging of the ONH and peripapillary RNFL was performed every other week under manometric IOP control (10 mm Hg) in 51 nonhuman primates (NHP) during baseline and after induction of unilateral EG. The ONH parameter minimum rim area (MRA) was derived from 80 radial B-scans centered on the ONH; RNFL cross-sectional area (RNFLA) from a peripapillary circular B-scan with 12° diameter. RESULTS: In control eyes, MRA was 1.00 ± 0.19 mm(2) at baseline and 1.00 ± 0.19 mm(2) at the final session (P = 0.77), while RNFLA was 0.95 ± 0.09 and 0.95 ± 0.10 mm(2), respectively (P = 0.96). In EG eyes, MRA decreased from 1.00 ± 0.19 mm(2) at baseline to 0.63 ± 0.21 mm(2) at the final session (P < 0.0001), while RNFLA decreased from 0.95 ± 0.09 to 0.74 ± 0.19 mm(2), respectively (P < 0.0001). Thus, MRA decreased by 36.4 ± 20.6% in EG eyes, significantly more than the decrease in RNFLA (21.7 ± 19.4%, P < 0.0001). Other significant changes in EG eyes included increased Bruch's membrane opening (BMO) nonplanarity (P < 0.05), decreased BMO aspect ratio (P < 0.0001), and decreased MRA angle (P < 0.001). Bruch's membrane opening area did not change from baseline in either control or EG eyes (P = 0.27, P = 0.15, respectively). CONCLUSIONS: Optic nerve head neural rim tissue thinning exceeded peripapillary RNFL thinning in NHP EG. These results support the hypothesis that axon bundles are compressed transversely within the ONH rim along with glaucomatous deformation of connective tissues. The Association for Research in Vision and Ophthalmology 2016-08-26 2016-08 /pmc/articles/PMC5016000/ /pubmed/27564522 http://dx.doi.org/10.1167/iovs.16-20000 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Glaucoma
Fortune, Brad
Reynaud, Juan
Hardin, Christy
Wang, Lin
Sigal, Ian A.
Burgoyne, Claude F.
Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title_full Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title_fullStr Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title_full_unstemmed Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title_short Experimental Glaucoma Causes Optic Nerve Head Neural Rim Tissue Compression: A Potentially Important Mechanism of Axon Injury
title_sort experimental glaucoma causes optic nerve head neural rim tissue compression: a potentially important mechanism of axon injury
topic Glaucoma
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016000/
https://www.ncbi.nlm.nih.gov/pubmed/27564522
http://dx.doi.org/10.1167/iovs.16-20000
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