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Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins
Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution of technologies capable of investigating peroxisomes directly in the cell. Here, we present the u...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016101/ https://www.ncbi.nlm.nih.gov/pubmed/27311714 http://dx.doi.org/10.1074/jbc.M116.734038 |
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author | Galiani, Silvia Waithe, Dominic Reglinski, Katharina Cruz-Zaragoza, Luis Daniel Garcia, Esther Clausen, Mathias P. Schliebs, Wolfgang Erdmann, Ralf Eggeling, Christian |
author_facet | Galiani, Silvia Waithe, Dominic Reglinski, Katharina Cruz-Zaragoza, Luis Daniel Garcia, Esther Clausen, Mathias P. Schliebs, Wolfgang Erdmann, Ralf Eggeling, Christian |
author_sort | Galiani, Silvia |
collection | PubMed |
description | Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution of technologies capable of investigating peroxisomes directly in the cell. Here, we present the use of super-resolution optical stimulated emission depletion microscopy to investigate with sub-60-nm resolution the heterogeneous spatial organization of the peroxisomal proteins PEX5, PEX14, and PEX11 around actively importing peroxisomes, showing distinct differences between these peroxins. Moreover, imported protein sterol carrier protein 2 (SCP2) occupies only a subregion of larger peroxisomes, highlighting the heterogeneous distribution of proteins even within the peroxisome. Finally, our data reveal subpopulations of peroxisomes showing only weak colocalization between PEX14 and PEX5 or PEX11 but at the same time a clear compartmentalized organization. This compartmentalization, which was less evident in cases of strong colocalization, indicates dynamic protein reorganization linked to changes occurring in the peroxisomes. Through the use of multicolor stimulated emission depletion microscopy, we have been able to characterize peroxisomes and their constituents to a yet unseen level of detail while maintaining a highly statistical approach, paving the way for equally complex biological studies in the future. |
format | Online Article Text |
id | pubmed-5016101 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-50161012016-09-12 Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins Galiani, Silvia Waithe, Dominic Reglinski, Katharina Cruz-Zaragoza, Luis Daniel Garcia, Esther Clausen, Mathias P. Schliebs, Wolfgang Erdmann, Ralf Eggeling, Christian J Biol Chem Cell Biology Membrane-associated events during peroxisomal protein import processes play an essential role in peroxisome functionality. Many details of these processes are not known due to missing spatial resolution of technologies capable of investigating peroxisomes directly in the cell. Here, we present the use of super-resolution optical stimulated emission depletion microscopy to investigate with sub-60-nm resolution the heterogeneous spatial organization of the peroxisomal proteins PEX5, PEX14, and PEX11 around actively importing peroxisomes, showing distinct differences between these peroxins. Moreover, imported protein sterol carrier protein 2 (SCP2) occupies only a subregion of larger peroxisomes, highlighting the heterogeneous distribution of proteins even within the peroxisome. Finally, our data reveal subpopulations of peroxisomes showing only weak colocalization between PEX14 and PEX5 or PEX11 but at the same time a clear compartmentalized organization. This compartmentalization, which was less evident in cases of strong colocalization, indicates dynamic protein reorganization linked to changes occurring in the peroxisomes. Through the use of multicolor stimulated emission depletion microscopy, we have been able to characterize peroxisomes and their constituents to a yet unseen level of detail while maintaining a highly statistical approach, paving the way for equally complex biological studies in the future. American Society for Biochemistry and Molecular Biology 2016-08-12 2016-06-16 /pmc/articles/PMC5016101/ /pubmed/27311714 http://dx.doi.org/10.1074/jbc.M116.734038 Text en © 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0) . |
spellingShingle | Cell Biology Galiani, Silvia Waithe, Dominic Reglinski, Katharina Cruz-Zaragoza, Luis Daniel Garcia, Esther Clausen, Mathias P. Schliebs, Wolfgang Erdmann, Ralf Eggeling, Christian Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title | Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title_full | Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title_fullStr | Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title_full_unstemmed | Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title_short | Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins |
title_sort | super-resolution microscopy reveals compartmentalization of peroxisomal membrane proteins |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016101/ https://www.ncbi.nlm.nih.gov/pubmed/27311714 http://dx.doi.org/10.1074/jbc.M116.734038 |
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