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Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats

INTRODUCTION: In this study, we aimed to determine the protective effects of hesperidin, a citrus flavonoid, in a model of testicular ischemia/reperfusion injury in rats. MATERIAL AND METHODS: Forty-two pubertal male Wistar-Albino rats were divided into six groups: group 1 – control; group 2 – 50 mg...

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Autores principales: Celik, Emrah, Oguzturk, Hakan, Sahin, Nurhan, Turtay, Muhammet Gökhan, Oguz, Fatih, Ciftci, Osman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016569/
https://www.ncbi.nlm.nih.gov/pubmed/27695481
http://dx.doi.org/10.5114/aoms.2015.47697
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author Celik, Emrah
Oguzturk, Hakan
Sahin, Nurhan
Turtay, Muhammet Gökhan
Oguz, Fatih
Ciftci, Osman
author_facet Celik, Emrah
Oguzturk, Hakan
Sahin, Nurhan
Turtay, Muhammet Gökhan
Oguz, Fatih
Ciftci, Osman
author_sort Celik, Emrah
collection PubMed
description INTRODUCTION: In this study, we aimed to determine the protective effects of hesperidin, a citrus flavonoid, in a model of testicular ischemia/reperfusion injury in rats. MATERIAL AND METHODS: Forty-two pubertal male Wistar-Albino rats were divided into six groups: group 1 – control; group 2 – 50 mg/kg hesperidin (low dose hesperidin) used without torsion (LH group); group 3 – 100 mg/kg hesperidin without torsion (HH group); group 4 – torsion/detorsion group (T/D); group 5 – T/D + 50 mg/kg hesperidin treatment group (T/D + LH); and group 6 – T/D + 100 mg/kg hesperidin treatment group (T/D + HH). Hesperidin was given to the treatment groups 30 min before detorsion. After the fourth hour of reperfusion, orchiectomy was performed on the rats under anesthesia. The tissue samples were examined histologically and biochemically. RESULTS: In the T/D group testicular malondialdehyde (MDA) levels were increased significantly (p < 0.001) whereas superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels were decreased compared to the control and other groups. However, hesperidin caused the effect of T/D to become closer to normal biochemical values. In addition, the histological examinations showed that T/D caused damage in the testis but hesperidin reduced this effect. The effects of hesperidin were found to be dose dependent. Thus, applying high doses would generate greater therapeutic effects. CONCLUSIONS: In a rat testicular T/D model we observed biochemical and histological damage due to ischemia. However, high and low dose applications of hesperidin were shown to have protective effects against this damage. Therefore, the aforementioned citrus flavonoid may provide positive results in cases of testicular torsion.
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spelling pubmed-50165692016-10-01 Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats Celik, Emrah Oguzturk, Hakan Sahin, Nurhan Turtay, Muhammet Gökhan Oguz, Fatih Ciftci, Osman Arch Med Sci Experimental Research INTRODUCTION: In this study, we aimed to determine the protective effects of hesperidin, a citrus flavonoid, in a model of testicular ischemia/reperfusion injury in rats. MATERIAL AND METHODS: Forty-two pubertal male Wistar-Albino rats were divided into six groups: group 1 – control; group 2 – 50 mg/kg hesperidin (low dose hesperidin) used without torsion (LH group); group 3 – 100 mg/kg hesperidin without torsion (HH group); group 4 – torsion/detorsion group (T/D); group 5 – T/D + 50 mg/kg hesperidin treatment group (T/D + LH); and group 6 – T/D + 100 mg/kg hesperidin treatment group (T/D + HH). Hesperidin was given to the treatment groups 30 min before detorsion. After the fourth hour of reperfusion, orchiectomy was performed on the rats under anesthesia. The tissue samples were examined histologically and biochemically. RESULTS: In the T/D group testicular malondialdehyde (MDA) levels were increased significantly (p < 0.001) whereas superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels were decreased compared to the control and other groups. However, hesperidin caused the effect of T/D to become closer to normal biochemical values. In addition, the histological examinations showed that T/D caused damage in the testis but hesperidin reduced this effect. The effects of hesperidin were found to be dose dependent. Thus, applying high doses would generate greater therapeutic effects. CONCLUSIONS: In a rat testicular T/D model we observed biochemical and histological damage due to ischemia. However, high and low dose applications of hesperidin were shown to have protective effects against this damage. Therefore, the aforementioned citrus flavonoid may provide positive results in cases of testicular torsion. Termedia Publishing House 2015-01-14 2016-10-01 /pmc/articles/PMC5016569/ /pubmed/27695481 http://dx.doi.org/10.5114/aoms.2015.47697 Text en Copyright: © 2015 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Experimental Research
Celik, Emrah
Oguzturk, Hakan
Sahin, Nurhan
Turtay, Muhammet Gökhan
Oguz, Fatih
Ciftci, Osman
Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title_full Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title_fullStr Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title_full_unstemmed Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title_short Protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
title_sort protective effects of hesperidin in experimental testicular ischemia/reperfusion injury in rats
topic Experimental Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016569/
https://www.ncbi.nlm.nih.gov/pubmed/27695481
http://dx.doi.org/10.5114/aoms.2015.47697
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