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Two-photon polymerized “nichoid” substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions
BACKGROUND: The use of pluripotent cells in stem cell therapy has major limitations, mainly related to the high costs and risks of exogenous conditioning and the use of feeder layers during cell expansion passages. METHODS: We developed an innovative three-dimensional culture substrate made of “nich...
Autores principales: | Nava, Michele M., Piuma, Alessio, Figliuzzi, Marina, Cattaneo, Irene, Bonandrini, Barbara, Zandrini, Tommaso, Cerullo, Giulio, Osellame, Roberto, Remuzzi, Andrea, Raimondi, Manuela T. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016857/ https://www.ncbi.nlm.nih.gov/pubmed/27613598 http://dx.doi.org/10.1186/s13287-016-0387-z |
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