Cargando…

Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae

Microalgae lipids are a promising energy source, but current biochemical methods of lipid-inductions such as nitrogen deprivation have low process robustness and controllability. Recently, use of mechanotransduction based membrane distortion by applying compression stress in a 2D-microsystem was sug...

Descripción completa

Detalles Bibliográficos
Autores principales: Cho, Myung Kwon, Shin, Hwa Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016897/
https://www.ncbi.nlm.nih.gov/pubmed/27609701
http://dx.doi.org/10.1038/srep32860
_version_ 1782452641652015104
author Cho, Myung Kwon
Shin, Hwa Sung
author_facet Cho, Myung Kwon
Shin, Hwa Sung
author_sort Cho, Myung Kwon
collection PubMed
description Microalgae lipids are a promising energy source, but current biochemical methods of lipid-inductions such as nitrogen deprivation have low process robustness and controllability. Recently, use of mechanotransduction based membrane distortion by applying compression stress in a 2D-microsystem was suggested as a way to overcome these limitations of biochemical induction. However, reproduction in large numbers of cells without cell death has been difficult to overcome because compression for direct membrane distortion reduces culture volume and leads to cell death due to nutrient deprivation. In this study, a mechanotransduction-induced lipid production (MDLP) system that redirects elastic microbeads to induce membrane distortion of microalgae with alleviating cell death was developed. This system resulted in accumulation of lipid in as little as 4 hr. Once compressed, porous microbeads absorb media and swell simultaneously while homogeneously inducing compression stress of microalgae. The absorbed media within beads could be supplied to adjacent cells and could minimize cell death from nutrient deficiency. All mechanotransduction was confirmed by measuring upregulation of calcium influx and Mat3 genes. The microbeads ensured robustness and controllability in repeated compression/de-compression processes. Overall, the MDLP system has potential for use as a fundamental biodiesel process that requires robustness and controllability.
format Online
Article
Text
id pubmed-5016897
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-50168972016-09-12 Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae Cho, Myung Kwon Shin, Hwa Sung Sci Rep Article Microalgae lipids are a promising energy source, but current biochemical methods of lipid-inductions such as nitrogen deprivation have low process robustness and controllability. Recently, use of mechanotransduction based membrane distortion by applying compression stress in a 2D-microsystem was suggested as a way to overcome these limitations of biochemical induction. However, reproduction in large numbers of cells without cell death has been difficult to overcome because compression for direct membrane distortion reduces culture volume and leads to cell death due to nutrient deprivation. In this study, a mechanotransduction-induced lipid production (MDLP) system that redirects elastic microbeads to induce membrane distortion of microalgae with alleviating cell death was developed. This system resulted in accumulation of lipid in as little as 4 hr. Once compressed, porous microbeads absorb media and swell simultaneously while homogeneously inducing compression stress of microalgae. The absorbed media within beads could be supplied to adjacent cells and could minimize cell death from nutrient deficiency. All mechanotransduction was confirmed by measuring upregulation of calcium influx and Mat3 genes. The microbeads ensured robustness and controllability in repeated compression/de-compression processes. Overall, the MDLP system has potential for use as a fundamental biodiesel process that requires robustness and controllability. Nature Publishing Group 2016-09-09 /pmc/articles/PMC5016897/ /pubmed/27609701 http://dx.doi.org/10.1038/srep32860 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cho, Myung Kwon
Shin, Hwa Sung
Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title_full Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title_fullStr Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title_full_unstemmed Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title_short Mechanotransduction-Induced Lipid Production System with High Robustness and Controllability for Microalgae
title_sort mechanotransduction-induced lipid production system with high robustness and controllability for microalgae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016897/
https://www.ncbi.nlm.nih.gov/pubmed/27609701
http://dx.doi.org/10.1038/srep32860
work_keys_str_mv AT chomyungkwon mechanotransductioninducedlipidproductionsystemwithhighrobustnessandcontrollabilityformicroalgae
AT shinhwasung mechanotransductioninducedlipidproductionsystemwithhighrobustnessandcontrollabilityformicroalgae