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Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment
Epigenetic modifications play crucial roles on establishment of tissue-specific transcription profiles and cellular characteristics. Direct conversions of fibroblasts into differentiated tissue cells by over-expression of critical transcription factors have been reported, but the epigenetic mechanis...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016969/ https://www.ncbi.nlm.nih.gov/pubmed/27608931 http://dx.doi.org/10.1038/srep32932 |
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author | Sekinaka, Tamotsu Hayashi, Yohei Noce, Toshiaki Niwa, Hitoshi Matsui, Yasuhisa |
author_facet | Sekinaka, Tamotsu Hayashi, Yohei Noce, Toshiaki Niwa, Hitoshi Matsui, Yasuhisa |
author_sort | Sekinaka, Tamotsu |
collection | PubMed |
description | Epigenetic modifications play crucial roles on establishment of tissue-specific transcription profiles and cellular characteristics. Direct conversions of fibroblasts into differentiated tissue cells by over-expression of critical transcription factors have been reported, but the epigenetic mechanisms underlying these conversions are still not fully understood. In addition, conversion of somatic cells into germ cells has not yet been achieved. To understand epigenetic mechanisms that underlie germ cell characteristics, we attempted to use defined epigenetic factors to directly convert mouse embryonic fibroblasts (MEFs) into germ cells. Here, we successfully induced germ cell-specific genes by inhibiting repressive epigenetic modifications via RNAi or small-molecule compounds. Under these conditions, some tissue-specific genes and stimulus-inducible genes were also induced. Meanwhile, the treatments did not result in genome-wide transcriptional activation. These results suggested that a permissive epigenetic environment resulted in selective de-repression of stimulus- and differentiation-inducible genes including germ cell-specific genes in MEFs. |
format | Online Article Text |
id | pubmed-5016969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50169692016-09-12 Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment Sekinaka, Tamotsu Hayashi, Yohei Noce, Toshiaki Niwa, Hitoshi Matsui, Yasuhisa Sci Rep Article Epigenetic modifications play crucial roles on establishment of tissue-specific transcription profiles and cellular characteristics. Direct conversions of fibroblasts into differentiated tissue cells by over-expression of critical transcription factors have been reported, but the epigenetic mechanisms underlying these conversions are still not fully understood. In addition, conversion of somatic cells into germ cells has not yet been achieved. To understand epigenetic mechanisms that underlie germ cell characteristics, we attempted to use defined epigenetic factors to directly convert mouse embryonic fibroblasts (MEFs) into germ cells. Here, we successfully induced germ cell-specific genes by inhibiting repressive epigenetic modifications via RNAi or small-molecule compounds. Under these conditions, some tissue-specific genes and stimulus-inducible genes were also induced. Meanwhile, the treatments did not result in genome-wide transcriptional activation. These results suggested that a permissive epigenetic environment resulted in selective de-repression of stimulus- and differentiation-inducible genes including germ cell-specific genes in MEFs. Nature Publishing Group 2016-09-09 /pmc/articles/PMC5016969/ /pubmed/27608931 http://dx.doi.org/10.1038/srep32932 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Sekinaka, Tamotsu Hayashi, Yohei Noce, Toshiaki Niwa, Hitoshi Matsui, Yasuhisa Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title | Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title_full | Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title_fullStr | Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title_full_unstemmed | Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title_short | Selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
title_sort | selective de-repression of germ cell-specific genes in mouse embryonic fibroblasts in a permissive epigenetic environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016969/ https://www.ncbi.nlm.nih.gov/pubmed/27608931 http://dx.doi.org/10.1038/srep32932 |
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