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In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments

BACKGROUND: There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host’s defense system. The key bacteria in biofilms are slow growing and support an attachm...

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Autores principales: Wu, Chongcong, Nakka, Sravya, Mansouri, Sepahdar, Bengtsson, Torbjörn, Nayeri, Tayeb, Nayeri, Fariba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017027/
https://www.ncbi.nlm.nih.gov/pubmed/27612600
http://dx.doi.org/10.1186/s12866-016-0821-5
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author Wu, Chongcong
Nakka, Sravya
Mansouri, Sepahdar
Bengtsson, Torbjörn
Nayeri, Tayeb
Nayeri, Fariba
author_facet Wu, Chongcong
Nakka, Sravya
Mansouri, Sepahdar
Bengtsson, Torbjörn
Nayeri, Tayeb
Nayeri, Fariba
author_sort Wu, Chongcong
collection PubMed
description BACKGROUND: There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host’s defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. Current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. The study aims to develop anti-bacterial human antibodies in vitro from patients who had recently undergone a systemic infection by pathogenic bacteria and to use these antibodies as a tool for detecting bacteria in biofilms. METHODS: Lymphocytes were separated from whole blood of patients (n = 10) and stimulated with heat-killed bacteria to produce antibodies in vitro. The specificity of antibodies in recognizing the bacteria against which they were directed was evaluated by surface plasmon resonance system (SPR) and electron microscopy. The ulcer secretions from patients with chronic and acute leg ulcers and healthy controls were analyzed by the SPR system and the results were compared with culture studies. RESULTS: The produced antibodies recognized bacteria with high sensitivity (SPR). The antibodies against Enterococcus fecalis bound specifically to the microorganism in a bacterial co-culture that was visualized by electron microscopy. CONCLUSION: In the present work, a method for producing specific antibodies against bacteria is introduced to recognize bacterial components in body fluids of patients suffering from pathogenic biofilms. This diagnostic technique may be most useful in clinical microbiology and in the choice of antibiotics in the treatment of serious infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-016-0821-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-50170272016-09-10 In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments Wu, Chongcong Nakka, Sravya Mansouri, Sepahdar Bengtsson, Torbjörn Nayeri, Tayeb Nayeri, Fariba BMC Microbiol Research Article BACKGROUND: There is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. Biofilms consist of polymicrobial communities that survive the host’s defense system. The key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. Current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. The study aims to develop anti-bacterial human antibodies in vitro from patients who had recently undergone a systemic infection by pathogenic bacteria and to use these antibodies as a tool for detecting bacteria in biofilms. METHODS: Lymphocytes were separated from whole blood of patients (n = 10) and stimulated with heat-killed bacteria to produce antibodies in vitro. The specificity of antibodies in recognizing the bacteria against which they were directed was evaluated by surface plasmon resonance system (SPR) and electron microscopy. The ulcer secretions from patients with chronic and acute leg ulcers and healthy controls were analyzed by the SPR system and the results were compared with culture studies. RESULTS: The produced antibodies recognized bacteria with high sensitivity (SPR). The antibodies against Enterococcus fecalis bound specifically to the microorganism in a bacterial co-culture that was visualized by electron microscopy. CONCLUSION: In the present work, a method for producing specific antibodies against bacteria is introduced to recognize bacterial components in body fluids of patients suffering from pathogenic biofilms. This diagnostic technique may be most useful in clinical microbiology and in the choice of antibiotics in the treatment of serious infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12866-016-0821-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-09 /pmc/articles/PMC5017027/ /pubmed/27612600 http://dx.doi.org/10.1186/s12866-016-0821-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Chongcong
Nakka, Sravya
Mansouri, Sepahdar
Bengtsson, Torbjörn
Nayeri, Tayeb
Nayeri, Fariba
In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title_full In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title_fullStr In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title_full_unstemmed In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title_short In vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
title_sort in vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017027/
https://www.ncbi.nlm.nih.gov/pubmed/27612600
http://dx.doi.org/10.1186/s12866-016-0821-5
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