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Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...
Autores principales: | Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017477/ https://www.ncbi.nlm.nih.gov/pubmed/27548185 http://dx.doi.org/10.3390/s16081312 |
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