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Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hy...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017730/ https://www.ncbi.nlm.nih.gov/pubmed/27611210 http://dx.doi.org/10.1371/journal.pone.0162418 |
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author | Vann, Nikolas C. Pham, Francis D. Hayes, John A. Kottick, Andrew Del Negro, Christopher A. |
author_facet | Vann, Nikolas C. Pham, Francis D. Hayes, John A. Kottick, Andrew Del Negro, Christopher A. |
author_sort | Vann, Nikolas C. |
collection | PubMed |
description | Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population. |
format | Online Article Text |
id | pubmed-5017730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-50177302016-09-27 Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice Vann, Nikolas C. Pham, Francis D. Hayes, John A. Kottick, Andrew Del Negro, Christopher A. PLoS One Research Article Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population. Public Library of Science 2016-09-09 /pmc/articles/PMC5017730/ /pubmed/27611210 http://dx.doi.org/10.1371/journal.pone.0162418 Text en © 2016 Vann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Vann, Nikolas C. Pham, Francis D. Hayes, John A. Kottick, Andrew Del Negro, Christopher A. Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title_full | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title_fullStr | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title_full_unstemmed | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title_short | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice |
title_sort | transient suppression of dbx1 prebötzinger interneurons disrupts breathing in adult mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017730/ https://www.ncbi.nlm.nih.gov/pubmed/27611210 http://dx.doi.org/10.1371/journal.pone.0162418 |
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