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Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice

Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hy...

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Autores principales: Vann, Nikolas C., Pham, Francis D., Hayes, John A., Kottick, Andrew, Del Negro, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017730/
https://www.ncbi.nlm.nih.gov/pubmed/27611210
http://dx.doi.org/10.1371/journal.pone.0162418
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author Vann, Nikolas C.
Pham, Francis D.
Hayes, John A.
Kottick, Andrew
Del Negro, Christopher A.
author_facet Vann, Nikolas C.
Pham, Francis D.
Hayes, John A.
Kottick, Andrew
Del Negro, Christopher A.
author_sort Vann, Nikolas C.
collection PubMed
description Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population.
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spelling pubmed-50177302016-09-27 Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice Vann, Nikolas C. Pham, Francis D. Hayes, John A. Kottick, Andrew Del Negro, Christopher A. PLoS One Research Article Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population. Public Library of Science 2016-09-09 /pmc/articles/PMC5017730/ /pubmed/27611210 http://dx.doi.org/10.1371/journal.pone.0162418 Text en © 2016 Vann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vann, Nikolas C.
Pham, Francis D.
Hayes, John A.
Kottick, Andrew
Del Negro, Christopher A.
Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title_full Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title_fullStr Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title_full_unstemmed Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title_short Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice
title_sort transient suppression of dbx1 prebötzinger interneurons disrupts breathing in adult mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017730/
https://www.ncbi.nlm.nih.gov/pubmed/27611210
http://dx.doi.org/10.1371/journal.pone.0162418
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