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High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines

Bread wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) has a complex allohexaploid genome, which makes it difficult to differentiate between the homoeologous sequences and assign them to the chromosome A, B, or D subgenomes. The chromosome-based draft genome sequence of the ‘Chinese Spring’ common wh...

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Autores principales: Zeng, Deying, Luo, Jiangtao, Li, Zenglin, Chen, Gang, Zhang, Lianquan, Ning, Shunzong, Yuan, Zhongwei, Zheng, Youliang, Hao, Ming, Liu, Dengcai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017740/
https://www.ncbi.nlm.nih.gov/pubmed/27611704
http://dx.doi.org/10.1371/journal.pone.0162847
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author Zeng, Deying
Luo, Jiangtao
Li, Zenglin
Chen, Gang
Zhang, Lianquan
Ning, Shunzong
Yuan, Zhongwei
Zheng, Youliang
Hao, Ming
Liu, Dengcai
author_facet Zeng, Deying
Luo, Jiangtao
Li, Zenglin
Chen, Gang
Zhang, Lianquan
Ning, Shunzong
Yuan, Zhongwei
Zheng, Youliang
Hao, Ming
Liu, Dengcai
author_sort Zeng, Deying
collection PubMed
description Bread wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) has a complex allohexaploid genome, which makes it difficult to differentiate between the homoeologous sequences and assign them to the chromosome A, B, or D subgenomes. The chromosome-based draft genome sequence of the ‘Chinese Spring’ common wheat cultivar enables the large-scale development of polymerase chain reaction (PCR)-based markers specific for homoeologs. Based on high-confidence ‘Chinese Spring’ genes with known functions, we developed 183 putative homoeolog-specific markers for chromosomes 4B and 7B. These markers were used in PCR assays for the 4B and 7B nullisomes and their euploid synthetic hexaploid wheat (SHW) line that was newly generated from a hybridization between Triticum turgidum (AABB) and the wild diploid species Aegilops tauschii (DD). Up to 64% of the markers for chromosomes 4B or 7B in the SHW background were confirmed to be homoeolog-specific. Thus, these markers were highly transferable between the ‘Chinese Spring’ bread wheat and SHW lines. Homoeolog-specific markers designed using genes with known functions may be useful for genetic investigations involving homoeologous chromosome tracking and homoeolog expression and interaction analyses.
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spelling pubmed-50177402016-09-27 High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines Zeng, Deying Luo, Jiangtao Li, Zenglin Chen, Gang Zhang, Lianquan Ning, Shunzong Yuan, Zhongwei Zheng, Youliang Hao, Ming Liu, Dengcai PLoS One Research Article Bread wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) has a complex allohexaploid genome, which makes it difficult to differentiate between the homoeologous sequences and assign them to the chromosome A, B, or D subgenomes. The chromosome-based draft genome sequence of the ‘Chinese Spring’ common wheat cultivar enables the large-scale development of polymerase chain reaction (PCR)-based markers specific for homoeologs. Based on high-confidence ‘Chinese Spring’ genes with known functions, we developed 183 putative homoeolog-specific markers for chromosomes 4B and 7B. These markers were used in PCR assays for the 4B and 7B nullisomes and their euploid synthetic hexaploid wheat (SHW) line that was newly generated from a hybridization between Triticum turgidum (AABB) and the wild diploid species Aegilops tauschii (DD). Up to 64% of the markers for chromosomes 4B or 7B in the SHW background were confirmed to be homoeolog-specific. Thus, these markers were highly transferable between the ‘Chinese Spring’ bread wheat and SHW lines. Homoeolog-specific markers designed using genes with known functions may be useful for genetic investigations involving homoeologous chromosome tracking and homoeolog expression and interaction analyses. Public Library of Science 2016-09-09 /pmc/articles/PMC5017740/ /pubmed/27611704 http://dx.doi.org/10.1371/journal.pone.0162847 Text en © 2016 Zeng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zeng, Deying
Luo, Jiangtao
Li, Zenglin
Chen, Gang
Zhang, Lianquan
Ning, Shunzong
Yuan, Zhongwei
Zheng, Youliang
Hao, Ming
Liu, Dengcai
High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title_full High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title_fullStr High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title_full_unstemmed High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title_short High Transferability of Homoeolog-Specific Markers between Bread Wheat and Newly Synthesized Hexaploid Wheat Lines
title_sort high transferability of homoeolog-specific markers between bread wheat and newly synthesized hexaploid wheat lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017740/
https://www.ncbi.nlm.nih.gov/pubmed/27611704
http://dx.doi.org/10.1371/journal.pone.0162847
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