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mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium

Neurogenesis is a key developmental event through which neurons are generated from neural stem/progenitor cells. Chromatin remodeling BAF (mSWI/SNF) complexes have been reported to play essential roles in the neurogenesis of the central nervous system. However, whether BAF complexes are required for...

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Autores principales: Bachmann, Christina, Nguyen, Huong, Rosenbusch, Joachim, Pham, Linh, Rabe, Tamara, Patwa, Megha, Sokpor, Godwin, Seong, Rho H., Ashery-Padan, Ruth, Mansouri, Ahmed, Stoykova, Anastassia, Staiger, Jochen F., Tuoc, Tran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017785/
https://www.ncbi.nlm.nih.gov/pubmed/27611684
http://dx.doi.org/10.1371/journal.pgen.1006274
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author Bachmann, Christina
Nguyen, Huong
Rosenbusch, Joachim
Pham, Linh
Rabe, Tamara
Patwa, Megha
Sokpor, Godwin
Seong, Rho H.
Ashery-Padan, Ruth
Mansouri, Ahmed
Stoykova, Anastassia
Staiger, Jochen F.
Tuoc, Tran
author_facet Bachmann, Christina
Nguyen, Huong
Rosenbusch, Joachim
Pham, Linh
Rabe, Tamara
Patwa, Megha
Sokpor, Godwin
Seong, Rho H.
Ashery-Padan, Ruth
Mansouri, Ahmed
Stoykova, Anastassia
Staiger, Jochen F.
Tuoc, Tran
author_sort Bachmann, Christina
collection PubMed
description Neurogenesis is a key developmental event through which neurons are generated from neural stem/progenitor cells. Chromatin remodeling BAF (mSWI/SNF) complexes have been reported to play essential roles in the neurogenesis of the central nervous system. However, whether BAF complexes are required for neuron generation in the olfactory system is unknown. Here, we identified onscBAF and ornBAF complexes, which are specifically present in olfactory neural stem cells (oNSCs) and olfactory receptor neurons (ORNs), respectively. We demonstrated that BAF155 subunit is highly expressed in both oNSCs and ORNs, whereas high expression of BAF170 subunit is observed only in ORNs. We report that conditional deletion of BAF155, a core subunit in both onscBAF and ornBAF complexes, causes impaired proliferation of oNSCs as well as defective maturation and axonogenesis of ORNs in the developing olfactory epithelium (OE), while the high expression of BAF170 is important for maturation of ORNs. Interestingly, in the absence of BAF complexes in BAF155/BAF170 double-conditional knockout mice (dcKO), OE is not specified. Mechanistically, BAF complex is required for normal activation of Pax6-dependent transcriptional activity in stem cells/progenitors of the OE. Our findings unveil a novel mechanism mediated by the mSWI/SNF complex in OE neurogenesis and development.
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spelling pubmed-50177852016-09-27 mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium Bachmann, Christina Nguyen, Huong Rosenbusch, Joachim Pham, Linh Rabe, Tamara Patwa, Megha Sokpor, Godwin Seong, Rho H. Ashery-Padan, Ruth Mansouri, Ahmed Stoykova, Anastassia Staiger, Jochen F. Tuoc, Tran PLoS Genet Research Article Neurogenesis is a key developmental event through which neurons are generated from neural stem/progenitor cells. Chromatin remodeling BAF (mSWI/SNF) complexes have been reported to play essential roles in the neurogenesis of the central nervous system. However, whether BAF complexes are required for neuron generation in the olfactory system is unknown. Here, we identified onscBAF and ornBAF complexes, which are specifically present in olfactory neural stem cells (oNSCs) and olfactory receptor neurons (ORNs), respectively. We demonstrated that BAF155 subunit is highly expressed in both oNSCs and ORNs, whereas high expression of BAF170 subunit is observed only in ORNs. We report that conditional deletion of BAF155, a core subunit in both onscBAF and ornBAF complexes, causes impaired proliferation of oNSCs as well as defective maturation and axonogenesis of ORNs in the developing olfactory epithelium (OE), while the high expression of BAF170 is important for maturation of ORNs. Interestingly, in the absence of BAF complexes in BAF155/BAF170 double-conditional knockout mice (dcKO), OE is not specified. Mechanistically, BAF complex is required for normal activation of Pax6-dependent transcriptional activity in stem cells/progenitors of the OE. Our findings unveil a novel mechanism mediated by the mSWI/SNF complex in OE neurogenesis and development. Public Library of Science 2016-09-09 /pmc/articles/PMC5017785/ /pubmed/27611684 http://dx.doi.org/10.1371/journal.pgen.1006274 Text en © 2016 Bachmann et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bachmann, Christina
Nguyen, Huong
Rosenbusch, Joachim
Pham, Linh
Rabe, Tamara
Patwa, Megha
Sokpor, Godwin
Seong, Rho H.
Ashery-Padan, Ruth
Mansouri, Ahmed
Stoykova, Anastassia
Staiger, Jochen F.
Tuoc, Tran
mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title_full mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title_fullStr mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title_full_unstemmed mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title_short mSWI/SNF (BAF) Complexes Are Indispensable for the Neurogenesis and Development of Embryonic Olfactory Epithelium
title_sort mswi/snf (baf) complexes are indispensable for the neurogenesis and development of embryonic olfactory epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017785/
https://www.ncbi.nlm.nih.gov/pubmed/27611684
http://dx.doi.org/10.1371/journal.pgen.1006274
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