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Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene

Previous transcriptome analyses have suggested that a gene cluster including a transcriptional regulator (blr7984) of the tetracycline repressor family was markedly down-regulated in symbiosis. Since blr7984 is annotated to be the transcriptional repressor, we hypothesized that it is involved in the...

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Autores principales: Ohkama-Ohtsu, Naoko, Honma, Haruna, Nakagome, Mariko, Nagata, Maki, Yamaya-Ito, Hiroko, Sano, Yoshiaki, Hiraoka, Norina, Ikemi, Takaaki, Suzuki, Akihiro, Okazaki, Shin, Minamisawa, Kiwamu, Yokoyama, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017801/
https://www.ncbi.nlm.nih.gov/pubmed/27383683
http://dx.doi.org/10.1264/jsme2.ME16056
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author Ohkama-Ohtsu, Naoko
Honma, Haruna
Nakagome, Mariko
Nagata, Maki
Yamaya-Ito, Hiroko
Sano, Yoshiaki
Hiraoka, Norina
Ikemi, Takaaki
Suzuki, Akihiro
Okazaki, Shin
Minamisawa, Kiwamu
Yokoyama, Tadashi
author_facet Ohkama-Ohtsu, Naoko
Honma, Haruna
Nakagome, Mariko
Nagata, Maki
Yamaya-Ito, Hiroko
Sano, Yoshiaki
Hiraoka, Norina
Ikemi, Takaaki
Suzuki, Akihiro
Okazaki, Shin
Minamisawa, Kiwamu
Yokoyama, Tadashi
author_sort Ohkama-Ohtsu, Naoko
collection PubMed
description Previous transcriptome analyses have suggested that a gene cluster including a transcriptional regulator (blr7984) of the tetracycline repressor family was markedly down-regulated in symbiosis. Since blr7984 is annotated to be the transcriptional repressor, we hypothesized that it is involved in the repression of genes in the genomic cluster including blr7984 in symbiotic bacteroids. In order to examine the function and involvement of the blr7984 gene in differentiation into bacteroids, we compared the free-living growth/symbiotic phenotype and gene expression between a blr7984-knockout mutant and the wild-type strain of Bradyrhizobium diazoefficiens USDA110. The mutant transiently increased the cell growth rate under free-living conditions and nodule numbers over those with the wild-type strain USDA110. The expression of three genes adjacent to the disrupted blr7984 gene was strongly up-regulated in the mutant in free-living and symbiotic cells. The mutant also induced the expression of genes for glutathione S-transferase, cytochrome c oxidases, ABC transporters, PTS sugar transport systems, and flagella synthesis under free-living conditions. bll7983 encoding glutathione S-transferase was up-regulated the most by the blr7984 disruption. Since redox regulation by glutathione is known to be involved in cell division in prokaryotes and eukaryotes, the strong expression of glutathione S-transferase encoded by the bll7983 gene may have caused redox changes in mutant cells, which resulted in higher rates of cell division.
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spelling pubmed-50178012016-09-12 Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene Ohkama-Ohtsu, Naoko Honma, Haruna Nakagome, Mariko Nagata, Maki Yamaya-Ito, Hiroko Sano, Yoshiaki Hiraoka, Norina Ikemi, Takaaki Suzuki, Akihiro Okazaki, Shin Minamisawa, Kiwamu Yokoyama, Tadashi Microbes Environ Articles Previous transcriptome analyses have suggested that a gene cluster including a transcriptional regulator (blr7984) of the tetracycline repressor family was markedly down-regulated in symbiosis. Since blr7984 is annotated to be the transcriptional repressor, we hypothesized that it is involved in the repression of genes in the genomic cluster including blr7984 in symbiotic bacteroids. In order to examine the function and involvement of the blr7984 gene in differentiation into bacteroids, we compared the free-living growth/symbiotic phenotype and gene expression between a blr7984-knockout mutant and the wild-type strain of Bradyrhizobium diazoefficiens USDA110. The mutant transiently increased the cell growth rate under free-living conditions and nodule numbers over those with the wild-type strain USDA110. The expression of three genes adjacent to the disrupted blr7984 gene was strongly up-regulated in the mutant in free-living and symbiotic cells. The mutant also induced the expression of genes for glutathione S-transferase, cytochrome c oxidases, ABC transporters, PTS sugar transport systems, and flagella synthesis under free-living conditions. bll7983 encoding glutathione S-transferase was up-regulated the most by the blr7984 disruption. Since redox regulation by glutathione is known to be involved in cell division in prokaryotes and eukaryotes, the strong expression of glutathione S-transferase encoded by the bll7983 gene may have caused redox changes in mutant cells, which resulted in higher rates of cell division. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2016-09 2016-07-05 /pmc/articles/PMC5017801/ /pubmed/27383683 http://dx.doi.org/10.1264/jsme2.ME16056 Text en Copyright © 2016 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Ohkama-Ohtsu, Naoko
Honma, Haruna
Nakagome, Mariko
Nagata, Maki
Yamaya-Ito, Hiroko
Sano, Yoshiaki
Hiraoka, Norina
Ikemi, Takaaki
Suzuki, Akihiro
Okazaki, Shin
Minamisawa, Kiwamu
Yokoyama, Tadashi
Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title_full Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title_fullStr Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title_full_unstemmed Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title_short Growth Rate of and Gene Expression in Bradyrhizobium diazoefficiens USDA110 due to a Mutation in blr7984, a TetR Family Transcriptional Regulator Gene
title_sort growth rate of and gene expression in bradyrhizobium diazoefficiens usda110 due to a mutation in blr7984, a tetr family transcriptional regulator gene
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5017801/
https://www.ncbi.nlm.nih.gov/pubmed/27383683
http://dx.doi.org/10.1264/jsme2.ME16056
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