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Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma

Nitrative and oxidative DNA damage plays an important role in inflammation-related carcinogenesis. To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor...

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Autores principales: Wang, Shumin, Ma, Ning, Zhao, Weilin, Midorikawa, Kaoru, Kawanishi, Shosuke, Hiraku, Yusuke, Oikawa, Shinji, Zhang, Zhe, Huang, Guangwu, Murata, Mariko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018333/
https://www.ncbi.nlm.nih.gov/pubmed/27647953
http://dx.doi.org/10.1155/2016/9343460
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author Wang, Shumin
Ma, Ning
Zhao, Weilin
Midorikawa, Kaoru
Kawanishi, Shosuke
Hiraku, Yusuke
Oikawa, Shinji
Zhang, Zhe
Huang, Guangwu
Murata, Mariko
author_facet Wang, Shumin
Ma, Ning
Zhao, Weilin
Midorikawa, Kaoru
Kawanishi, Shosuke
Hiraku, Yusuke
Oikawa, Shinji
Zhang, Zhe
Huang, Guangwu
Murata, Mariko
author_sort Wang, Shumin
collection PubMed
description Nitrative and oxidative DNA damage plays an important role in inflammation-related carcinogenesis. To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. We also measured 8-nitroguanine formation as an indicator of inflammation-related DNA lesions. The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC.
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spelling pubmed-50183332016-09-19 Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma Wang, Shumin Ma, Ning Zhao, Weilin Midorikawa, Kaoru Kawanishi, Shosuke Hiraku, Yusuke Oikawa, Shinji Zhang, Zhe Huang, Guangwu Murata, Mariko Mediators Inflamm Research Article Nitrative and oxidative DNA damage plays an important role in inflammation-related carcinogenesis. To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. We also measured 8-nitroguanine formation as an indicator of inflammation-related DNA lesions. The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. Similarly, more intense staining of CD44v6 and ALDH1A1 was detected in an NPC cell line than in an immortalized nasopharyngeal epithelial cell line. In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. Hindawi Publishing Corporation 2016 2016-08-28 /pmc/articles/PMC5018333/ /pubmed/27647953 http://dx.doi.org/10.1155/2016/9343460 Text en Copyright © 2016 Shumin Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Shumin
Ma, Ning
Zhao, Weilin
Midorikawa, Kaoru
Kawanishi, Shosuke
Hiraku, Yusuke
Oikawa, Shinji
Zhang, Zhe
Huang, Guangwu
Murata, Mariko
Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title_full Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title_fullStr Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title_full_unstemmed Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title_short Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
title_sort inflammation-related dna damage and cancer stem cell markers in nasopharyngeal carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018333/
https://www.ncbi.nlm.nih.gov/pubmed/27647953
http://dx.doi.org/10.1155/2016/9343460
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