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Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo

The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia le...

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Autores principales: Yun, Chanyong, Jung, Youngchul, Chun, Wonjoo, Yang, Beodeul, Ryu, Junghyun, Lim, Chiyeon, Kim, Jung-Hoon, Kim, Hyungwoo, Cho, Su-In
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018339/
https://www.ncbi.nlm.nih.gov/pubmed/27647952
http://dx.doi.org/10.1155/2016/8027537
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author Yun, Chanyong
Jung, Youngchul
Chun, Wonjoo
Yang, Beodeul
Ryu, Junghyun
Lim, Chiyeon
Kim, Jung-Hoon
Kim, Hyungwoo
Cho, Su-In
author_facet Yun, Chanyong
Jung, Youngchul
Chun, Wonjoo
Yang, Beodeul
Ryu, Junghyun
Lim, Chiyeon
Kim, Jung-Hoon
Kim, Hyungwoo
Cho, Su-In
author_sort Yun, Chanyong
collection PubMed
description The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia leaf extract (ALE) using CD mice and Raw 264.7 cells. The effects of ALE on histopathological changes and cytokine production in ear tissues were assessed in mice with CD induced by 1-fluoro-2,4-dinitrobenzene (DNFB). Moreover, the anti-inflammatory effects on production levels of prostaglandin E(2) (PGE(2)) and nitric oxide (NO) and expression levels of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) were investigated in Raw 264.7 cells. Topical application of ALE effectively prevented ear swelling induced by repeated DNFB application. ALE prevented epidermal hyperplasia and infiltration of immune cells and lowered the production of interferon- (IFN-) gamma (γ), tumour necrosis factor- (TNF-) alpha (α), and interleukin- (IL-) 6 in inflamed tissues. In addition, ALE inhibited expression of COX-2 and iNOS and production of NO and PGE(2) in Raw 264.7 cells. These results indicate that Artemisia leaf can be used as a therapeutic agent for inflammatory skin diseases and that its anti-inflammatory effects are closely related to the inhibition of inflammatory mediator release from macrophages and inflammatory cytokine production in inflamed tissues.
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spelling pubmed-50183392016-09-19 Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo Yun, Chanyong Jung, Youngchul Chun, Wonjoo Yang, Beodeul Ryu, Junghyun Lim, Chiyeon Kim, Jung-Hoon Kim, Hyungwoo Cho, Su-In Mediators Inflamm Research Article The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia leaf extract (ALE) using CD mice and Raw 264.7 cells. The effects of ALE on histopathological changes and cytokine production in ear tissues were assessed in mice with CD induced by 1-fluoro-2,4-dinitrobenzene (DNFB). Moreover, the anti-inflammatory effects on production levels of prostaglandin E(2) (PGE(2)) and nitric oxide (NO) and expression levels of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) were investigated in Raw 264.7 cells. Topical application of ALE effectively prevented ear swelling induced by repeated DNFB application. ALE prevented epidermal hyperplasia and infiltration of immune cells and lowered the production of interferon- (IFN-) gamma (γ), tumour necrosis factor- (TNF-) alpha (α), and interleukin- (IL-) 6 in inflamed tissues. In addition, ALE inhibited expression of COX-2 and iNOS and production of NO and PGE(2) in Raw 264.7 cells. These results indicate that Artemisia leaf can be used as a therapeutic agent for inflammatory skin diseases and that its anti-inflammatory effects are closely related to the inhibition of inflammatory mediator release from macrophages and inflammatory cytokine production in inflamed tissues. Hindawi Publishing Corporation 2016 2016-08-28 /pmc/articles/PMC5018339/ /pubmed/27647952 http://dx.doi.org/10.1155/2016/8027537 Text en Copyright © 2016 Chanyong Yun et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yun, Chanyong
Jung, Youngchul
Chun, Wonjoo
Yang, Beodeul
Ryu, Junghyun
Lim, Chiyeon
Kim, Jung-Hoon
Kim, Hyungwoo
Cho, Su-In
Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title_full Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title_fullStr Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title_full_unstemmed Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title_short Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
title_sort anti-inflammatory effects of artemisia leaf extract in mice with contact dermatitis in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018339/
https://www.ncbi.nlm.nih.gov/pubmed/27647952
http://dx.doi.org/10.1155/2016/8027537
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