Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture condit...

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Detalles Bibliográficos
Autores principales: Merritt, Jason, Kuehn, Seppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018735/
https://www.ncbi.nlm.nih.gov/pubmed/27616752
http://dx.doi.org/10.1038/srep33173
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author Merritt, Jason
Kuehn, Seppe
author_facet Merritt, Jason
Kuehn, Seppe
author_sort Merritt, Jason
collection PubMed
description We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.
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spelling pubmed-50187352016-09-19 Quantitative high-throughput population dynamics in continuous-culture by automated microscopy Merritt, Jason Kuehn, Seppe Sci Rep Article We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation. Nature Publishing Group 2016-09-12 /pmc/articles/PMC5018735/ /pubmed/27616752 http://dx.doi.org/10.1038/srep33173 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Merritt, Jason
Kuehn, Seppe
Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title_full Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title_fullStr Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title_full_unstemmed Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title_short Quantitative high-throughput population dynamics in continuous-culture by automated microscopy
title_sort quantitative high-throughput population dynamics in continuous-culture by automated microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018735/
https://www.ncbi.nlm.nih.gov/pubmed/27616752
http://dx.doi.org/10.1038/srep33173
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