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Regulation of cell-cell fusion by nanotopography
Cell-cell fusion is fundamental to a multitude of biological processes ranging from cell differentiation and embryogenesis to cancer metastasis and biomaterial-tissue interactions. Fusogenic cells are exposed to biochemical and biophysical factors, which could potentially alter cell behavior. While...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018837/ https://www.ncbi.nlm.nih.gov/pubmed/27615159 http://dx.doi.org/10.1038/srep33277 |
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author | Padmanabhan, Jagannath Augelli, Michael J. Cheung, Bettina Kinser, Emily R. Cleary, Barnett Kumar, Priyanka Wang, Renhao Sawyer, Andrew J. Li, Rui Schwarz, Udo D. Schroers, Jan Kyriakides, Themis R. |
author_facet | Padmanabhan, Jagannath Augelli, Michael J. Cheung, Bettina Kinser, Emily R. Cleary, Barnett Kumar, Priyanka Wang, Renhao Sawyer, Andrew J. Li, Rui Schwarz, Udo D. Schroers, Jan Kyriakides, Themis R. |
author_sort | Padmanabhan, Jagannath |
collection | PubMed |
description | Cell-cell fusion is fundamental to a multitude of biological processes ranging from cell differentiation and embryogenesis to cancer metastasis and biomaterial-tissue interactions. Fusogenic cells are exposed to biochemical and biophysical factors, which could potentially alter cell behavior. While biochemical inducers of fusion such as cytokines and kinases have been identified, little is known about the biophysical regulation of cell-cell fusion. Here, we designed experiments to examine cell-cell fusion using bulk metallic glass (BMG) nanorod arrays with varying biophysical cues, i.e. nanotopography and stiffness. Through independent variation of stiffness and topography, we found that nanotopography constitutes the primary biophysical cue that can override biochemical signals to attenuate fusion. Specifically, nanotopography restricts cytoskeletal remodeling-associated signaling, which leads to reduced fusion. This finding expands our fundamental understanding of the nanoscale biophysical regulation of cell fusion and can be exploited in biomaterials design to induce desirable biomaterial-tissue interactions. |
format | Online Article Text |
id | pubmed-5018837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50188372016-09-19 Regulation of cell-cell fusion by nanotopography Padmanabhan, Jagannath Augelli, Michael J. Cheung, Bettina Kinser, Emily R. Cleary, Barnett Kumar, Priyanka Wang, Renhao Sawyer, Andrew J. Li, Rui Schwarz, Udo D. Schroers, Jan Kyriakides, Themis R. Sci Rep Article Cell-cell fusion is fundamental to a multitude of biological processes ranging from cell differentiation and embryogenesis to cancer metastasis and biomaterial-tissue interactions. Fusogenic cells are exposed to biochemical and biophysical factors, which could potentially alter cell behavior. While biochemical inducers of fusion such as cytokines and kinases have been identified, little is known about the biophysical regulation of cell-cell fusion. Here, we designed experiments to examine cell-cell fusion using bulk metallic glass (BMG) nanorod arrays with varying biophysical cues, i.e. nanotopography and stiffness. Through independent variation of stiffness and topography, we found that nanotopography constitutes the primary biophysical cue that can override biochemical signals to attenuate fusion. Specifically, nanotopography restricts cytoskeletal remodeling-associated signaling, which leads to reduced fusion. This finding expands our fundamental understanding of the nanoscale biophysical regulation of cell fusion and can be exploited in biomaterials design to induce desirable biomaterial-tissue interactions. Nature Publishing Group 2016-09-12 /pmc/articles/PMC5018837/ /pubmed/27615159 http://dx.doi.org/10.1038/srep33277 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Padmanabhan, Jagannath Augelli, Michael J. Cheung, Bettina Kinser, Emily R. Cleary, Barnett Kumar, Priyanka Wang, Renhao Sawyer, Andrew J. Li, Rui Schwarz, Udo D. Schroers, Jan Kyriakides, Themis R. Regulation of cell-cell fusion by nanotopography |
title | Regulation of cell-cell fusion by nanotopography |
title_full | Regulation of cell-cell fusion by nanotopography |
title_fullStr | Regulation of cell-cell fusion by nanotopography |
title_full_unstemmed | Regulation of cell-cell fusion by nanotopography |
title_short | Regulation of cell-cell fusion by nanotopography |
title_sort | regulation of cell-cell fusion by nanotopography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018837/ https://www.ncbi.nlm.nih.gov/pubmed/27615159 http://dx.doi.org/10.1038/srep33277 |
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