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Design methodology for a confocal imaging system using an objective microlens array with an increased working distance
In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. Thi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018843/ https://www.ncbi.nlm.nih.gov/pubmed/27615370 http://dx.doi.org/10.1038/srep33278 |
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author | Choi, Woojae Shin, Ryung Lim, Jiseok Kang, Shinill |
author_facet | Choi, Woojae Shin, Ryung Lim, Jiseok Kang, Shinill |
author_sort | Choi, Woojae |
collection | PubMed |
description | In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. This system overcomes the limitations of the optical complexities of conventional multi-optical probe confocal imaging systems and the short working distance using a micro-objective lens module composed of two microlens arrays and a telecentric relay optical system. The micro-objective lens module was fabricated on a glass substrate using backside alignment photolithography and thermal reflow processes. To test the feasibility of the developed methodology, an optical system with a resolution of 1 μm/pixel using multi-optical probes with an array size of 10 × 10 was designed and constructed. The developed system provides a 1 mm × 1 mm field of view and a sample scanning range of 100 μm. The optical resolution was evaluated by conducting sample tests using a knife-edge detecting method. The measured lateral resolution of the system was 0.98 μm. |
format | Online Article Text |
id | pubmed-5018843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50188432016-09-19 Design methodology for a confocal imaging system using an objective microlens array with an increased working distance Choi, Woojae Shin, Ryung Lim, Jiseok Kang, Shinill Sci Rep Article In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. This system overcomes the limitations of the optical complexities of conventional multi-optical probe confocal imaging systems and the short working distance using a micro-objective lens module composed of two microlens arrays and a telecentric relay optical system. The micro-objective lens module was fabricated on a glass substrate using backside alignment photolithography and thermal reflow processes. To test the feasibility of the developed methodology, an optical system with a resolution of 1 μm/pixel using multi-optical probes with an array size of 10 × 10 was designed and constructed. The developed system provides a 1 mm × 1 mm field of view and a sample scanning range of 100 μm. The optical resolution was evaluated by conducting sample tests using a knife-edge detecting method. The measured lateral resolution of the system was 0.98 μm. Nature Publishing Group 2016-09-12 /pmc/articles/PMC5018843/ /pubmed/27615370 http://dx.doi.org/10.1038/srep33278 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Choi, Woojae Shin, Ryung Lim, Jiseok Kang, Shinill Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title | Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title_full | Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title_fullStr | Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title_full_unstemmed | Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title_short | Design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
title_sort | design methodology for a confocal imaging system using an objective microlens array with an increased working distance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018843/ https://www.ncbi.nlm.nih.gov/pubmed/27615370 http://dx.doi.org/10.1038/srep33278 |
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