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Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s
Since the initial identification of cytochrome P450 monooxygenases (CYPs/P450s), great progress has been made in understanding their structure-function relationship, diversity and application in producing compounds beneficial to humans. However, the molecular evolution of P450s in terms of their dyn...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018878/ https://www.ncbi.nlm.nih.gov/pubmed/27616185 http://dx.doi.org/10.1038/srep33099 |
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author | Parvez, Mohammad Qhanya, Lehlohonolo Benedict Mthakathi, Ntsane Trevor Kgosiemang, Ipeleng Kopano Rosinah Bamal, Hans Denis Pagadala, Nataraj Sekhar Xie, Ting Yang, Haoran Chen, Hengye Theron, Chrispian William Monyaki, Richie Raselemane, Seiso Caiphus Salewe, Vuyani Mongale, Bogadi Lorato Matowane, Retshedisitswe Godfrey Abdalla, Sara Mohamed Hasaan Booi, Wool Isaac van Wyk, Mari Olivier, Dedré Boucher, Charlotte E. Nelson, David R. Tuszynski, Jack A. Blackburn, Jonathan Michael Yu, Jae-Hyuk Mashele, Samson Sitheni Chen, Wanping Syed, Khajamohiddin |
author_facet | Parvez, Mohammad Qhanya, Lehlohonolo Benedict Mthakathi, Ntsane Trevor Kgosiemang, Ipeleng Kopano Rosinah Bamal, Hans Denis Pagadala, Nataraj Sekhar Xie, Ting Yang, Haoran Chen, Hengye Theron, Chrispian William Monyaki, Richie Raselemane, Seiso Caiphus Salewe, Vuyani Mongale, Bogadi Lorato Matowane, Retshedisitswe Godfrey Abdalla, Sara Mohamed Hasaan Booi, Wool Isaac van Wyk, Mari Olivier, Dedré Boucher, Charlotte E. Nelson, David R. Tuszynski, Jack A. Blackburn, Jonathan Michael Yu, Jae-Hyuk Mashele, Samson Sitheni Chen, Wanping Syed, Khajamohiddin |
author_sort | Parvez, Mohammad |
collection | PubMed |
description | Since the initial identification of cytochrome P450 monooxygenases (CYPs/P450s), great progress has been made in understanding their structure-function relationship, diversity and application in producing compounds beneficial to humans. However, the molecular evolution of P450s in terms of their dynamics both at protein and DNA levels and functional conservation across kingdoms still needs investigation. In this study, we analyzed 17 598 P450s belonging to 113 P450 families (bacteria −42; fungi −19; plant −28; animal −22; plant and animal −1 and common P450 family −1) and found highly conserved and rapidly evolving P450 families. Results suggested that bacterial P450s, particularly P450s belonging to mycobacteria, are highly conserved both at protein and DNA levels. Mycobacteria possess the highest P450 diversity percentage compared to other microbes and have a high coverage of P450s (≥1%) in their genomes, as found in fungi and plants. Phylogenetic and functional analyses revealed the functional conservation of P450s despite belonging to different biological kingdoms, suggesting the adherence of P450s to their innate function such as their involvement in either generation or oxidation of steroids and structurally related molecules, fatty acids and terpenoids. This study’s results offer new understanding of the dynamic structural nature of P450s. |
format | Online Article Text |
id | pubmed-5018878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50188782016-09-19 Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s Parvez, Mohammad Qhanya, Lehlohonolo Benedict Mthakathi, Ntsane Trevor Kgosiemang, Ipeleng Kopano Rosinah Bamal, Hans Denis Pagadala, Nataraj Sekhar Xie, Ting Yang, Haoran Chen, Hengye Theron, Chrispian William Monyaki, Richie Raselemane, Seiso Caiphus Salewe, Vuyani Mongale, Bogadi Lorato Matowane, Retshedisitswe Godfrey Abdalla, Sara Mohamed Hasaan Booi, Wool Isaac van Wyk, Mari Olivier, Dedré Boucher, Charlotte E. Nelson, David R. Tuszynski, Jack A. Blackburn, Jonathan Michael Yu, Jae-Hyuk Mashele, Samson Sitheni Chen, Wanping Syed, Khajamohiddin Sci Rep Article Since the initial identification of cytochrome P450 monooxygenases (CYPs/P450s), great progress has been made in understanding their structure-function relationship, diversity and application in producing compounds beneficial to humans. However, the molecular evolution of P450s in terms of their dynamics both at protein and DNA levels and functional conservation across kingdoms still needs investigation. In this study, we analyzed 17 598 P450s belonging to 113 P450 families (bacteria −42; fungi −19; plant −28; animal −22; plant and animal −1 and common P450 family −1) and found highly conserved and rapidly evolving P450 families. Results suggested that bacterial P450s, particularly P450s belonging to mycobacteria, are highly conserved both at protein and DNA levels. Mycobacteria possess the highest P450 diversity percentage compared to other microbes and have a high coverage of P450s (≥1%) in their genomes, as found in fungi and plants. Phylogenetic and functional analyses revealed the functional conservation of P450s despite belonging to different biological kingdoms, suggesting the adherence of P450s to their innate function such as their involvement in either generation or oxidation of steroids and structurally related molecules, fatty acids and terpenoids. This study’s results offer new understanding of the dynamic structural nature of P450s. Nature Publishing Group 2016-09-12 /pmc/articles/PMC5018878/ /pubmed/27616185 http://dx.doi.org/10.1038/srep33099 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Parvez, Mohammad Qhanya, Lehlohonolo Benedict Mthakathi, Ntsane Trevor Kgosiemang, Ipeleng Kopano Rosinah Bamal, Hans Denis Pagadala, Nataraj Sekhar Xie, Ting Yang, Haoran Chen, Hengye Theron, Chrispian William Monyaki, Richie Raselemane, Seiso Caiphus Salewe, Vuyani Mongale, Bogadi Lorato Matowane, Retshedisitswe Godfrey Abdalla, Sara Mohamed Hasaan Booi, Wool Isaac van Wyk, Mari Olivier, Dedré Boucher, Charlotte E. Nelson, David R. Tuszynski, Jack A. Blackburn, Jonathan Michael Yu, Jae-Hyuk Mashele, Samson Sitheni Chen, Wanping Syed, Khajamohiddin Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title | Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title_full | Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title_fullStr | Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title_full_unstemmed | Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title_short | Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s |
title_sort | molecular evolutionary dynamics of cytochrome p450 monooxygenases across kingdoms: special focus on mycobacterial p450s |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018878/ https://www.ncbi.nlm.nih.gov/pubmed/27616185 http://dx.doi.org/10.1038/srep33099 |
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