Deep Sequencing of Random Mutant Libraries Reveals the Active Site of the Narrow Specificity CphA Metallo-β-Lactamase is Fragile to Mutations

CphA is a Zn(2+)-dependent metallo-β-lactamase that efficiently hydrolyzes only carbapenem antibiotics. To understand the sequence requirements for CphA function, single codon random mutant libraries were constructed for residues in and near the active site and mutants were selected for E. coli grow...

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Detalles Bibliográficos
Autores principales: Sun, Zhizeng, Mehta, Shrenik C., Adamski, Carolyn J., Gibbs, Richard A., Palzkill, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018959/
https://www.ncbi.nlm.nih.gov/pubmed/27616327
http://dx.doi.org/10.1038/srep33195

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5018959/
https://www.ncbi.nlm.nih.gov/pubmed/27616327
http://dx.doi.org/10.1038/srep33195

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