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Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field
The potential of the application of pulsed electric fields (PEF) to induce accelerate autolysis of a commercial strain of Saccharomyces cerevisiae for winemaking use was evaluated. The influence of PEF treatments of different intensity (5–25 kV/cm for 30–240 μs) on cell viability, cytoplasmic membra...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019107/ https://www.ncbi.nlm.nih.gov/pubmed/27672386 http://dx.doi.org/10.3389/fmicb.2016.01435 |
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author | Martínez, Juan M. Cebrián, Guillermo Álvarez, Ignacio Raso, Javier |
author_facet | Martínez, Juan M. Cebrián, Guillermo Álvarez, Ignacio Raso, Javier |
author_sort | Martínez, Juan M. |
collection | PubMed |
description | The potential of the application of pulsed electric fields (PEF) to induce accelerate autolysis of a commercial strain of Saccharomyces cerevisiae for winemaking use was evaluated. The influence of PEF treatments of different intensity (5–25 kV/cm for 30–240 μs) on cell viability, cytoplasmic membrane permeabilization and release of mannoproteins and compounds absorbing at 260 and 280 nm has been investigated. After 8 days of incubation at 25°C the Abs(600) of the suspension containing the control cells was kept constant while the Abs(600) of the suspension containing the cells treated by PEF decreased. The measurement of the absorbance at 260 and 280 nm revealed no release of UV absorbing material from untreated cells after 8 days of incubation but the amount of UV absorbing material released drastically increased in the samples that contained cells treated by PEF after the same storage period. After 18 days of storage the amount of mannoproteins released from the untreated cell was negligible. Conversely, mannoprotein concentration increased linearly for the samples containing cells of S. cerevisiae treated by PEF. After 18 days of incubation the concentration of mannoproteins in the supernatant increased 4.2 times for the samples containing cells treated by PEF at 15 and 25 kV/cm for 45 and 150 μs. Results obtained in this study indicates that PEF could be used in winemaking to accelerate the sur lie aging or to obtain mannoproteins from yeast cultures. |
format | Online Article Text |
id | pubmed-5019107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50191072016-09-26 Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field Martínez, Juan M. Cebrián, Guillermo Álvarez, Ignacio Raso, Javier Front Microbiol Microbiology The potential of the application of pulsed electric fields (PEF) to induce accelerate autolysis of a commercial strain of Saccharomyces cerevisiae for winemaking use was evaluated. The influence of PEF treatments of different intensity (5–25 kV/cm for 30–240 μs) on cell viability, cytoplasmic membrane permeabilization and release of mannoproteins and compounds absorbing at 260 and 280 nm has been investigated. After 8 days of incubation at 25°C the Abs(600) of the suspension containing the control cells was kept constant while the Abs(600) of the suspension containing the cells treated by PEF decreased. The measurement of the absorbance at 260 and 280 nm revealed no release of UV absorbing material from untreated cells after 8 days of incubation but the amount of UV absorbing material released drastically increased in the samples that contained cells treated by PEF after the same storage period. After 18 days of storage the amount of mannoproteins released from the untreated cell was negligible. Conversely, mannoprotein concentration increased linearly for the samples containing cells of S. cerevisiae treated by PEF. After 18 days of incubation the concentration of mannoproteins in the supernatant increased 4.2 times for the samples containing cells treated by PEF at 15 and 25 kV/cm for 45 and 150 μs. Results obtained in this study indicates that PEF could be used in winemaking to accelerate the sur lie aging or to obtain mannoproteins from yeast cultures. Frontiers Media S.A. 2016-09-12 /pmc/articles/PMC5019107/ /pubmed/27672386 http://dx.doi.org/10.3389/fmicb.2016.01435 Text en Copyright © 2016 Martínez, Cebrián, Álvarez and Raso. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Martínez, Juan M. Cebrián, Guillermo Álvarez, Ignacio Raso, Javier Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title_full | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title_fullStr | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title_full_unstemmed | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title_short | Release of Mannoproteins during Saccharomyces cerevisiae Autolysis Induced by Pulsed Electric Field |
title_sort | release of mannoproteins during saccharomyces cerevisiae autolysis induced by pulsed electric field |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019107/ https://www.ncbi.nlm.nih.gov/pubmed/27672386 http://dx.doi.org/10.3389/fmicb.2016.01435 |
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