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Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei

Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their saf...

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Autores principales: Tavakoli, Yasaman, Esmaeili, Abolghasem, Rabbani, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019208/
https://www.ncbi.nlm.nih.gov/pubmed/27844008
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author Tavakoli, Yasaman
Esmaeili, Abolghasem
Rabbani, Mohammad
author_facet Tavakoli, Yasaman
Esmaeili, Abolghasem
Rabbani, Mohammad
author_sort Tavakoli, Yasaman
collection PubMed
description Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5′-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria.
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spelling pubmed-50192082016-11-14 Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad Mol Biol Res Commun Short Communication Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5′-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria. Shiraz University 2015-09 /pmc/articles/PMC5019208/ /pubmed/27844008 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Tavakoli, Yasaman
Esmaeili, Abolghasem
Rabbani, Mohammad
Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title_full Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title_fullStr Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title_full_unstemmed Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title_short Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
title_sort identification and molecular cloning of glutamate decarboxylase gene from lactobacillus casei
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019208/
https://www.ncbi.nlm.nih.gov/pubmed/27844008
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