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Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei
Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their saf...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019208/ https://www.ncbi.nlm.nih.gov/pubmed/27844008 |
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author | Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad |
author_facet | Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad |
author_sort | Tavakoli, Yasaman |
collection | PubMed |
description | Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5′-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria. |
format | Online Article Text |
id | pubmed-5019208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Shiraz University |
record_format | MEDLINE/PubMed |
spelling | pubmed-50192082016-11-14 Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad Mol Biol Res Commun Short Communication Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5′-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria. Shiraz University 2015-09 /pmc/articles/PMC5019208/ /pubmed/27844008 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title | Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title_full | Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title_fullStr | Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title_full_unstemmed | Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title_short | Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei |
title_sort | identification and molecular cloning of glutamate decarboxylase gene from lactobacillus casei |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019208/ https://www.ncbi.nlm.nih.gov/pubmed/27844008 |
work_keys_str_mv | AT tavakoliyasaman identificationandmolecularcloningofglutamatedecarboxylasegenefromlactobacilluscasei AT esmaeiliabolghasem identificationandmolecularcloningofglutamatedecarboxylasegenefromlactobacilluscasei AT rabbanimohammad identificationandmolecularcloningofglutamatedecarboxylasegenefromlactobacilluscasei |