Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)

The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the...

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Autores principales: Abdolahnejad, Zeinab, Pourkazemi, Mohammad, Khoshkholgh, Majid Reza, Yarmohammadi, Mahtab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019210/
https://www.ncbi.nlm.nih.gov/pubmed/27844010
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author Abdolahnejad, Zeinab
Pourkazemi, Mohammad
Khoshkholgh, Majid Reza
Yarmohammadi, Mahtab
author_facet Abdolahnejad, Zeinab
Pourkazemi, Mohammad
Khoshkholgh, Majid Reza
Yarmohammadi, Mahtab
author_sort Abdolahnejad, Zeinab
collection PubMed
description The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.
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spelling pubmed-50192102016-11-14 Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii) Abdolahnejad, Zeinab Pourkazemi, Mohammad Khoshkholgh, Majid Reza Yarmohammadi, Mahtab Mol Biol Res Commun Original Article The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon. Shiraz University 2015-12 /pmc/articles/PMC5019210/ /pubmed/27844010 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Abdolahnejad, Zeinab
Pourkazemi, Mohammad
Khoshkholgh, Majid Reza
Yarmohammadi, Mahtab
Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title_full Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title_fullStr Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title_full_unstemmed Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title_short Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
title_sort expression of growth hormone gene during early development of siberian sturgeon (acipenser baerii)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019210/
https://www.ncbi.nlm.nih.gov/pubmed/27844010
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