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3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus
PURPOSE: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. MATERIALS...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019273/ https://www.ncbi.nlm.nih.gov/pubmed/27660439 http://dx.doi.org/10.2147/IJN.S111880 |
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author | Demin, Alexander M Pershina, Alexandra G Ivanov, Vladimir V Nevskaya, Kseniya V Shevelev, Oleg B Minin, Artyom S Byzov, Iliya V Sazonov, Alexey E Krasnov, Victor P Ogorodova, Ludmila M |
author_facet | Demin, Alexander M Pershina, Alexandra G Ivanov, Vladimir V Nevskaya, Kseniya V Shevelev, Oleg B Minin, Artyom S Byzov, Iliya V Sazonov, Alexey E Krasnov, Victor P Ogorodova, Ludmila M |
author_sort | Demin, Alexander M |
collection | PubMed |
description | PURPOSE: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. MATERIALS AND METHODS: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe(3+) and Fe(2+) salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic resonance properties of MNPs were investigated in vitro and in vivo. RESULTS: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g(−1)) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r(2) was 271 mmol(−1) s(−1). To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus. CONCLUSION: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage. |
format | Online Article Text |
id | pubmed-5019273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-50192732016-09-22 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus Demin, Alexander M Pershina, Alexandra G Ivanov, Vladimir V Nevskaya, Kseniya V Shevelev, Oleg B Minin, Artyom S Byzov, Iliya V Sazonov, Alexey E Krasnov, Victor P Ogorodova, Ludmila M Int J Nanomedicine Original Research PURPOSE: Liver fluke causes severe liver damage in an infected human. However, the infection often remains neglected due to the lack of pathognomonic signs. Nanoparticle-enhanced magnetic resonance imaging (MRI) offers a promising technique for detecting liver lesions induced by parasites. MATERIALS AND METHODS: Surface modification of iron oxide nanoparticles produced by coprecipitation from a solution of Fe(3+) and Fe(2+) salts using 3-aminopropylsilane (APS) was carried out. The APS-modified nanoparticles were characterized by transmission electron microscopy, fourier transform infrared spectroscopy, and thermogravimetric analysis. Magnetic resonance properties of MNPs were investigated in vitro and in vivo. RESULTS: The amount of APS grafted on the surface of nanoparticles (0.60±0.06 mmol g(−1)) was calculated based on elemental analysis and infrared spectroscopy data. According to transmission electron microscopy data, there were no essential changes in the structure of nanoparticles during the modification. The APS-modified nanoparticles exhibit high magnetic properties; the calculated relaxivity r(2) was 271 mmol(−1) s(−1). To obtain suspension with optimal hydrodynamic characteristics, amino groups on the surface of nanoparticles were converted into an ionic form with HCl. Cellular uptake of modified nanoparticles by rat hepatoma cells and human monocytes in vitro was 74.1±4.5 and 10.0±3.7 pg [Fe] per cell, respectively. Low cytotoxicity of the nanoparticles was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Annexin V/7-aminoactinomycin D flow cytometry assays. For the first time, magnetic nanoparticles were applied for contrast-enhanced MRI of liver lesions induced by Opisthorchis felineus. CONCLUSION: The synthesized APS-modified iron oxide nanoparticles showed high efficiency as an MRI contrast agent for the evaluation of opisthorchiasis-related liver damage. Dove Medical Press 2016-09-06 /pmc/articles/PMC5019273/ /pubmed/27660439 http://dx.doi.org/10.2147/IJN.S111880 Text en © 2016 Demin et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Demin, Alexander M Pershina, Alexandra G Ivanov, Vladimir V Nevskaya, Kseniya V Shevelev, Oleg B Minin, Artyom S Byzov, Iliya V Sazonov, Alexey E Krasnov, Victor P Ogorodova, Ludmila M 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title | 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title_full | 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title_fullStr | 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title_full_unstemmed | 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title_short | 3-Aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by Opisthorchis felineus |
title_sort | 3-aminopropylsilane-modified iron oxide nanoparticles for contrast-enhanced magnetic resonance imaging of liver lesions induced by opisthorchis felineus |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5019273/ https://www.ncbi.nlm.nih.gov/pubmed/27660439 http://dx.doi.org/10.2147/IJN.S111880 |
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