Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus

BACKGROUND: The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes...

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Autores principales: Yan, Wei, Liu, Li, Qin, Weiquan, Luo, Youqing, Ma, Xuezhong, Haider, Nabil, Inayeh, Muhanad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020018/
https://www.ncbi.nlm.nih.gov/pubmed/27652115
http://dx.doi.org/10.1186/s40064-016-3198-x
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author Yan, Wei
Liu, Li
Qin, Weiquan
Luo, Youqing
Ma, Xuezhong
Haider, Nabil
Inayeh, Muhanad
author_facet Yan, Wei
Liu, Li
Qin, Weiquan
Luo, Youqing
Ma, Xuezhong
Haider, Nabil
Inayeh, Muhanad
author_sort Yan, Wei
collection PubMed
description BACKGROUND: The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes (RferOBP1-11) were identified. FINDINGS: The result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively. CONCLUSIONS: The results paved the way towards a future understanding of the olfaction in this species.
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spelling pubmed-50200182016-09-20 Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus Yan, Wei Liu, Li Qin, Weiquan Luo, Youqing Ma, Xuezhong Haider, Nabil Inayeh, Muhanad Springerplus Short Report BACKGROUND: The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes (RferOBP1-11) were identified. FINDINGS: The result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively. CONCLUSIONS: The results paved the way towards a future understanding of the olfaction in this species. Springer International Publishing 2016-09-13 /pmc/articles/PMC5020018/ /pubmed/27652115 http://dx.doi.org/10.1186/s40064-016-3198-x Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Report
Yan, Wei
Liu, Li
Qin, Weiquan
Luo, Youqing
Ma, Xuezhong
Haider, Nabil
Inayeh, Muhanad
Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title_full Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title_fullStr Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title_full_unstemmed Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title_short Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus
title_sort identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, rhynchophorus ferrugineus
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020018/
https://www.ncbi.nlm.nih.gov/pubmed/27652115
http://dx.doi.org/10.1186/s40064-016-3198-x
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