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Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini

BACKGROUND: Paragonimiasis is an important and widespread neglected tropical disease. Fifteen Paragonimus species are human pathogens, but two of these, Paragonimus westermani and P. skrjabini, are responsible for the bulk of human disease. Despite their medical and economic significance, there is l...

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Autores principales: Li, Ben-wen, McNulty, Samantha N., Rosa, Bruce A., Tyagi, Rahul, Zeng, Qing Ren, Gu, Kong-zhen, Weil, Gary J., Mitreva, Makedonka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020434/
https://www.ncbi.nlm.nih.gov/pubmed/27619014
http://dx.doi.org/10.1186/s13071-016-1785-x
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author Li, Ben-wen
McNulty, Samantha N.
Rosa, Bruce A.
Tyagi, Rahul
Zeng, Qing Ren
Gu, Kong-zhen
Weil, Gary J.
Mitreva, Makedonka
author_facet Li, Ben-wen
McNulty, Samantha N.
Rosa, Bruce A.
Tyagi, Rahul
Zeng, Qing Ren
Gu, Kong-zhen
Weil, Gary J.
Mitreva, Makedonka
author_sort Li, Ben-wen
collection PubMed
description BACKGROUND: Paragonimiasis is an important and widespread neglected tropical disease. Fifteen Paragonimus species are human pathogens, but two of these, Paragonimus westermani and P. skrjabini, are responsible for the bulk of human disease. Despite their medical and economic significance, there is limited information on the gene content and expression of Paragonimus lung flukes. RESULTS: The transcriptomes of adult P. westermani and P. skrjabini were studied with deep sequencing technology. Approximately 30 million reads per species were assembled into 21,586 and 25,825 unigenes for P. westermani and P. skrjabini, respectively. Many unigenes showed homology with sequences from other food-borne trematodes, but 1,217 high-confidence Paragonimus-specific unigenes were identified. Analyses indicated that both species have the potential for aerobic and anaerobic metabolism but not de novo fatty acid biosynthesis and that they may interact with host signaling pathways. Some 12,432 P. westermani and P. skrjabini unigenes showed a clear correspondence in bi-directional sequence similarity matches. The expression of shared unigenes was mostly well correlated, but differentially expressed unigenes were identified and shown to be enriched for functions related to proteolysis for P. westermani and microtubule based motility for P. skrjabini. CONCLUSIONS: The assembled transcriptomes of P. westermani and P. skrjabini, inferred proteins, and extensive functional annotations generated for this project (including identified primary sequence similarities to various species, protein domains, biological pathways, predicted proteases, molecular mimics and secreted proteins, etc.) represent a valuable resource for hypothesis driven research on these medically and economically important species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1785-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-50204342016-09-14 Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini Li, Ben-wen McNulty, Samantha N. Rosa, Bruce A. Tyagi, Rahul Zeng, Qing Ren Gu, Kong-zhen Weil, Gary J. Mitreva, Makedonka Parasit Vectors Research BACKGROUND: Paragonimiasis is an important and widespread neglected tropical disease. Fifteen Paragonimus species are human pathogens, but two of these, Paragonimus westermani and P. skrjabini, are responsible for the bulk of human disease. Despite their medical and economic significance, there is limited information on the gene content and expression of Paragonimus lung flukes. RESULTS: The transcriptomes of adult P. westermani and P. skrjabini were studied with deep sequencing technology. Approximately 30 million reads per species were assembled into 21,586 and 25,825 unigenes for P. westermani and P. skrjabini, respectively. Many unigenes showed homology with sequences from other food-borne trematodes, but 1,217 high-confidence Paragonimus-specific unigenes were identified. Analyses indicated that both species have the potential for aerobic and anaerobic metabolism but not de novo fatty acid biosynthesis and that they may interact with host signaling pathways. Some 12,432 P. westermani and P. skrjabini unigenes showed a clear correspondence in bi-directional sequence similarity matches. The expression of shared unigenes was mostly well correlated, but differentially expressed unigenes were identified and shown to be enriched for functions related to proteolysis for P. westermani and microtubule based motility for P. skrjabini. CONCLUSIONS: The assembled transcriptomes of P. westermani and P. skrjabini, inferred proteins, and extensive functional annotations generated for this project (including identified primary sequence similarities to various species, protein domains, biological pathways, predicted proteases, molecular mimics and secreted proteins, etc.) represent a valuable resource for hypothesis driven research on these medically and economically important species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1785-x) contains supplementary material, which is available to authorized users. BioMed Central 2016-09-13 /pmc/articles/PMC5020434/ /pubmed/27619014 http://dx.doi.org/10.1186/s13071-016-1785-x Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Li, Ben-wen
McNulty, Samantha N.
Rosa, Bruce A.
Tyagi, Rahul
Zeng, Qing Ren
Gu, Kong-zhen
Weil, Gary J.
Mitreva, Makedonka
Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title_full Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title_fullStr Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title_full_unstemmed Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title_short Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini
title_sort conservation and diversification of the transcriptomes of adult paragonimus westermani and p. skrjabini
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020434/
https://www.ncbi.nlm.nih.gov/pubmed/27619014
http://dx.doi.org/10.1186/s13071-016-1785-x
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