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EGFP oligomers as natural fluorescence and hydrodynamic standards

EGFP oligomers are convenient standards for experiments on fluorescent protein-tagged biomolecules. In this study, we characterized their hydrodynamic and fluorescence properties. Diffusion coefficients D of EGFP(1–4) were determined by analytical ultracentrifugation with fluorescence detection and...

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Detalles Bibliográficos
Autores principales: Vámosi, György, Mücke, Norbert, Müller, Gabriele, Krieger, Jan Wolfgang, Curth, Ute, Langowski, Jörg, Tóth, Katalin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020695/
https://www.ncbi.nlm.nih.gov/pubmed/27622431
http://dx.doi.org/10.1038/srep33022