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EGFP oligomers as natural fluorescence and hydrodynamic standards
EGFP oligomers are convenient standards for experiments on fluorescent protein-tagged biomolecules. In this study, we characterized their hydrodynamic and fluorescence properties. Diffusion coefficients D of EGFP(1–4) were determined by analytical ultracentrifugation with fluorescence detection and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020695/ https://www.ncbi.nlm.nih.gov/pubmed/27622431 http://dx.doi.org/10.1038/srep33022 |