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Automatic counting of microglial cell activation and its applications

Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion cells. This disease results in vision loss and blindness. Any vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early d...

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Detalles Bibliográficos
Autores principales: Gallego, Beatriz I., de Gracia, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020808/
https://www.ncbi.nlm.nih.gov/pubmed/27651757
http://dx.doi.org/10.4103/1673-5374.189166
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author Gallego, Beatriz I.
de Gracia, Pablo
author_facet Gallego, Beatriz I.
de Gracia, Pablo
author_sort Gallego, Beatriz I.
collection PubMed
description Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion cells. This disease results in vision loss and blindness. Any vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early detection and treatment, could offer neuronal protection and avoid later serious damages to the visual function. A full understanding of the etiology of the disease will still require the contribution of many scientific efforts. Glial activation has been observed in glaucoma, being microglial proliferation a hallmark in this neurodegenerative disease. A typical project studying these cellular changes involved in glaucoma often needs thousands of images - from several animals - covering different layers and regions of the retina. The gold standard to evaluate them is the manual count. This method requires a large amount of time from specialized personnel. It is a tedious process and prone to human error. We present here a new method to count microglial cells by using a computer algorithm. It counts in one hour the same number of images that a researcher counts in four weeks, with no loss of reliability.
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spelling pubmed-50208082016-09-20 Automatic counting of microglial cell activation and its applications Gallego, Beatriz I. de Gracia, Pablo Neural Regen Res Invited Review Glaucoma is a multifactorial optic neuropathy characterized by the damage and death of the retinal ganglion cells. This disease results in vision loss and blindness. Any vision loss resulting from the disease cannot be restored and nowadays there is no available cure for glaucoma; however an early detection and treatment, could offer neuronal protection and avoid later serious damages to the visual function. A full understanding of the etiology of the disease will still require the contribution of many scientific efforts. Glial activation has been observed in glaucoma, being microglial proliferation a hallmark in this neurodegenerative disease. A typical project studying these cellular changes involved in glaucoma often needs thousands of images - from several animals - covering different layers and regions of the retina. The gold standard to evaluate them is the manual count. This method requires a large amount of time from specialized personnel. It is a tedious process and prone to human error. We present here a new method to count microglial cells by using a computer algorithm. It counts in one hour the same number of images that a researcher counts in four weeks, with no loss of reliability. Medknow Publications & Media Pvt Ltd 2016-08 /pmc/articles/PMC5020808/ /pubmed/27651757 http://dx.doi.org/10.4103/1673-5374.189166 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Invited Review
Gallego, Beatriz I.
de Gracia, Pablo
Automatic counting of microglial cell activation and its applications
title Automatic counting of microglial cell activation and its applications
title_full Automatic counting of microglial cell activation and its applications
title_fullStr Automatic counting of microglial cell activation and its applications
title_full_unstemmed Automatic counting of microglial cell activation and its applications
title_short Automatic counting of microglial cell activation and its applications
title_sort automatic counting of microglial cell activation and its applications
topic Invited Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5020808/
https://www.ncbi.nlm.nih.gov/pubmed/27651757
http://dx.doi.org/10.4103/1673-5374.189166
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