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Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer

Our recent study of the microRNA (miRNA) expression signature of bladder cancer (BC) by deep‐sequencing revealed that two miRNA, microRNA‐139‐5p/microRNA‐139‐3p were significantly downregulated in BC tissues. The aim of this study was to investigate the functional roles of these miRNA and their modu...

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Autores principales: Yonemori, Masaya, Seki, Naohiko, Yoshino, Hirofumi, Matsushita, Ryosuke, Miyamoto, Kazutaka, Nakagawa, Masayuki, Enokida, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021030/
https://www.ncbi.nlm.nih.gov/pubmed/27355528
http://dx.doi.org/10.1111/cas.13002
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author Yonemori, Masaya
Seki, Naohiko
Yoshino, Hirofumi
Matsushita, Ryosuke
Miyamoto, Kazutaka
Nakagawa, Masayuki
Enokida, Hideki
author_facet Yonemori, Masaya
Seki, Naohiko
Yoshino, Hirofumi
Matsushita, Ryosuke
Miyamoto, Kazutaka
Nakagawa, Masayuki
Enokida, Hideki
author_sort Yonemori, Masaya
collection PubMed
description Our recent study of the microRNA (miRNA) expression signature of bladder cancer (BC) by deep‐sequencing revealed that two miRNA, microRNA‐139‐5p/microRNA‐139‐3p were significantly downregulated in BC tissues. The aim of this study was to investigate the functional roles of these miRNA and their modulation of cancer networks in BC cells. Functional assays of BC cells were performed using transfection of mature miRNA or small interfering RNA (siRNA). Genome‐wide gene expression analysis, in silico analysis and dual‐luciferase reporter assays were applied to identify miRNA targets. The associations between the expression of miRNA and its targets and overall survival were estimated by the Kaplan–Meier method. Gain‐of‐function studies showed that miR‐139‐5p and miR‐139‐3p significantly inhibited cell migration and invasion by BC cells. The matrix metalloprotease 11 gene (MMP11) was identified as a direct target of miR‐139‐5p and miR‐139‐3p. Kaplan–Meier survival curves showed that higher expression of MMP11 predicted shorter survival of BC patients (P = 0.029). Downregulated miR‐139‐5p or miR‐139‐3p enhanced BC cell migration and invasion in BC cells. MMP11 was directly regulated by these miRNA and might be a good prognostic marker for survival of BC patients.
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spelling pubmed-50210302016-09-20 Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer Yonemori, Masaya Seki, Naohiko Yoshino, Hirofumi Matsushita, Ryosuke Miyamoto, Kazutaka Nakagawa, Masayuki Enokida, Hideki Cancer Sci Original Articles Our recent study of the microRNA (miRNA) expression signature of bladder cancer (BC) by deep‐sequencing revealed that two miRNA, microRNA‐139‐5p/microRNA‐139‐3p were significantly downregulated in BC tissues. The aim of this study was to investigate the functional roles of these miRNA and their modulation of cancer networks in BC cells. Functional assays of BC cells were performed using transfection of mature miRNA or small interfering RNA (siRNA). Genome‐wide gene expression analysis, in silico analysis and dual‐luciferase reporter assays were applied to identify miRNA targets. The associations between the expression of miRNA and its targets and overall survival were estimated by the Kaplan–Meier method. Gain‐of‐function studies showed that miR‐139‐5p and miR‐139‐3p significantly inhibited cell migration and invasion by BC cells. The matrix metalloprotease 11 gene (MMP11) was identified as a direct target of miR‐139‐5p and miR‐139‐3p. Kaplan–Meier survival curves showed that higher expression of MMP11 predicted shorter survival of BC patients (P = 0.029). Downregulated miR‐139‐5p or miR‐139‐3p enhanced BC cell migration and invasion in BC cells. MMP11 was directly regulated by these miRNA and might be a good prognostic marker for survival of BC patients. John Wiley and Sons Inc. 2016-09-06 2016-09 /pmc/articles/PMC5021030/ /pubmed/27355528 http://dx.doi.org/10.1111/cas.13002 Text en © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Yonemori, Masaya
Seki, Naohiko
Yoshino, Hirofumi
Matsushita, Ryosuke
Miyamoto, Kazutaka
Nakagawa, Masayuki
Enokida, Hideki
Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title_full Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title_fullStr Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title_full_unstemmed Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title_short Dual tumor‐suppressors miR‐139‐5p and miR‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
title_sort dual tumor‐suppressors mir‐139‐5p and mir‐139‐3p targeting matrix metalloprotease 11 in bladder cancer
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021030/
https://www.ncbi.nlm.nih.gov/pubmed/27355528
http://dx.doi.org/10.1111/cas.13002
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