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The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

Sutherlandia frutescens is a medicinal plant indigenous to Southern Africa and is commonly known as the “cancer bush.” This plant has traditionally been used for the treatment of various ailments, although it is best known for its claims of activity against “internal” cancers. Here we report on its...

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Detalles Bibliográficos
Autores principales: van der Walt, Nicola B., Zakeri, Zahra, Cronjé, Marianne J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021500/
https://www.ncbi.nlm.nih.gov/pubmed/27656236
http://dx.doi.org/10.1155/2016/4921067
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author van der Walt, Nicola B.
Zakeri, Zahra
Cronjé, Marianne J.
author_facet van der Walt, Nicola B.
Zakeri, Zahra
Cronjé, Marianne J.
author_sort van der Walt, Nicola B.
collection PubMed
description Sutherlandia frutescens is a medicinal plant indigenous to Southern Africa and is commonly known as the “cancer bush.” This plant has traditionally been used for the treatment of various ailments, although it is best known for its claims of activity against “internal” cancers. Here we report on its effect on melanoma cells. The aim of this study was to investigate whether an extract of S. frutescens could induce apoptosis in the A375 melanoma cell line and to outline the basic mechanism of action. S. frutescens extract induced apoptosis in A375 cells as evidenced by morphological features of apoptosis, phosphatidylserine exposure, nuclear condensation, caspase activation, and the release of cytochrome c from the mitochondria. Studies in the presence of a pan-caspase inhibitor allude to caspase-independent cell death, which appeared to be mediated by the apoptosis inducing factor. Taken together, the results of this study show that S. frutescens extract is effective in inducing apoptosis in malignant melanoma cells and indicates that further in vivo mechanistic studies may be warranted.
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spelling pubmed-50215002016-09-21 The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens van der Walt, Nicola B. Zakeri, Zahra Cronjé, Marianne J. Evid Based Complement Alternat Med Research Article Sutherlandia frutescens is a medicinal plant indigenous to Southern Africa and is commonly known as the “cancer bush.” This plant has traditionally been used for the treatment of various ailments, although it is best known for its claims of activity against “internal” cancers. Here we report on its effect on melanoma cells. The aim of this study was to investigate whether an extract of S. frutescens could induce apoptosis in the A375 melanoma cell line and to outline the basic mechanism of action. S. frutescens extract induced apoptosis in A375 cells as evidenced by morphological features of apoptosis, phosphatidylserine exposure, nuclear condensation, caspase activation, and the release of cytochrome c from the mitochondria. Studies in the presence of a pan-caspase inhibitor allude to caspase-independent cell death, which appeared to be mediated by the apoptosis inducing factor. Taken together, the results of this study show that S. frutescens extract is effective in inducing apoptosis in malignant melanoma cells and indicates that further in vivo mechanistic studies may be warranted. Hindawi Publishing Corporation 2016 2016-08-30 /pmc/articles/PMC5021500/ /pubmed/27656236 http://dx.doi.org/10.1155/2016/4921067 Text en Copyright © 2016 Nicola B. van der Walt et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
van der Walt, Nicola B.
Zakeri, Zahra
Cronjé, Marianne J.
The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title_full The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title_fullStr The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title_full_unstemmed The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title_short The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens
title_sort induction of apoptosis in a375 malignant melanoma cells by sutherlandia frutescens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021500/
https://www.ncbi.nlm.nih.gov/pubmed/27656236
http://dx.doi.org/10.1155/2016/4921067
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