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In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis

Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G. duodenalis genome: GdDMC1A and GdDMC1B. In this article, we describe the...

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Autores principales: Torres-Huerta, Ana Laura, Martínez-Miguel, Rosa María, Bazán-Tejeda, María Luisa, Bermúdez-Cruz, Rosa María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021851/
https://www.ncbi.nlm.nih.gov/pubmed/27660811
http://dx.doi.org/10.1016/j.dib.2016.08.031
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author Torres-Huerta, Ana Laura
Martínez-Miguel, Rosa María
Bazán-Tejeda, María Luisa
Bermúdez-Cruz, Rosa María
author_facet Torres-Huerta, Ana Laura
Martínez-Miguel, Rosa María
Bazán-Tejeda, María Luisa
Bermúdez-Cruz, Rosa María
author_sort Torres-Huerta, Ana Laura
collection PubMed
description Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G. duodenalis genome: GdDMC1A and GdDMC1B. In this article, we describe the identification of conserved domains in GdDMC1A and GdDMC1B, such as: DNA binding domains (Helix-turn-helix motif, loops 1 and 2) and an ATPcap and Walker A and B motifs associated with ATP binding and hydrolysis, phylogenetic analyses among assemblages and three-dimensional structure modeling of these recombinases using bioinformatics tools. Also, experimental data is described about LD50 determination for ionizing radiation in trophozoites of G. duodenalis. Additionally, as recombinases, GdDMC1A and GdDMC1B were used to rescue a defective Saccharomyces cerevisiae Δ rad51 strain under genotoxic conditions and data is described. The data described here are related to the research article entitled “Characterization of recombinase DMC1B and its functional role as Rad51 in DNA damage repair in Giardia duodenalis trophozoites” (Torres-Huerta et al.,) [1].
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spelling pubmed-50218512016-09-22 In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis Torres-Huerta, Ana Laura Martínez-Miguel, Rosa María Bazán-Tejeda, María Luisa Bermúdez-Cruz, Rosa María Data Brief Data Article Giardia duodenalis is a worldwide protozoa known causing diarrhea in all vertebrates, humans among these. Homologous recombination is a mechanism that provides genomic stability. Two putative recombinases were identified in G. duodenalis genome: GdDMC1A and GdDMC1B. In this article, we describe the identification of conserved domains in GdDMC1A and GdDMC1B, such as: DNA binding domains (Helix-turn-helix motif, loops 1 and 2) and an ATPcap and Walker A and B motifs associated with ATP binding and hydrolysis, phylogenetic analyses among assemblages and three-dimensional structure modeling of these recombinases using bioinformatics tools. Also, experimental data is described about LD50 determination for ionizing radiation in trophozoites of G. duodenalis. Additionally, as recombinases, GdDMC1A and GdDMC1B were used to rescue a defective Saccharomyces cerevisiae Δ rad51 strain under genotoxic conditions and data is described. The data described here are related to the research article entitled “Characterization of recombinase DMC1B and its functional role as Rad51 in DNA damage repair in Giardia duodenalis trophozoites” (Torres-Huerta et al.,) [1]. Elsevier 2016-08-22 /pmc/articles/PMC5021851/ /pubmed/27660811 http://dx.doi.org/10.1016/j.dib.2016.08.031 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Torres-Huerta, Ana Laura
Martínez-Miguel, Rosa María
Bazán-Tejeda, María Luisa
Bermúdez-Cruz, Rosa María
In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title_full In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title_fullStr In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title_full_unstemmed In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title_short In silico data analyses of recombinases GdDMC1A and GdDMC1B from Giardia duodenalis
title_sort in silico data analyses of recombinases gddmc1a and gddmc1b from giardia duodenalis
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021851/
https://www.ncbi.nlm.nih.gov/pubmed/27660811
http://dx.doi.org/10.1016/j.dib.2016.08.031
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