Cargando…
Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013)
Aim. To gain insight into the molecular diversity of sapovirus in outpatients with acute gastroenteritis in Nanjing, China. Methods. The specimens from outpatients clinically diagnosed as acute gastroenteritis were detected by real-time PCR; RT-PCR was then performed to amplify part of VP1 sequences...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021866/ https://www.ncbi.nlm.nih.gov/pubmed/27656204 http://dx.doi.org/10.1155/2016/4210462 |
_version_ | 1782453409485422592 |
---|---|
author | Zhang, Hong-ying Qiao, Meng-kai Wang, Xuan He, Min Shi, Li-min Xie, Guo-xiang Jin, Hei-ying |
author_facet | Zhang, Hong-ying Qiao, Meng-kai Wang, Xuan He, Min Shi, Li-min Xie, Guo-xiang Jin, Hei-ying |
author_sort | Zhang, Hong-ying |
collection | PubMed |
description | Aim. To gain insight into the molecular diversity of sapovirus in outpatients with acute gastroenteritis in Nanjing, China. Methods. The specimens from outpatients clinically diagnosed as acute gastroenteritis were detected by real-time PCR; RT-PCR was then performed to amplify part of VP1 sequences. The PCR products were cloned into pGEM-T Easy vector and bidirectionally sequenced. All sequences were edited and analyzed. A phylogenetic tree was drawn with the MEGA 5.0 software. Results. Between 2011 and 2013, 16 sapovirus positive cases were confirmed by real-time PCR. The infected cases increased from two in 2011 and six in 2012 to eight in 2013. The majority was children and the elderly (15, 93.75%) and single infections (15, 93.75%). Of the 16 real-time positive specimens, 14 specimens had PCR products and the analysis data of the 14 nucleic sequences showed that there was one GI genogroup with four genotypes, two GI.2 in 2011, three GI.2, and one GI.1 in 2012 and one GI.2, three GI.1, two GI.3, and two GI.5 in 2013. Conclusion. Our data confirmed continuous existing of GI genogroup and GI.2 genotype from 2011 to 2013 in Nanjing and the successive appearance of different genotypes from outpatients with gastroenteritis. |
format | Online Article Text |
id | pubmed-5021866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-50218662016-09-21 Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) Zhang, Hong-ying Qiao, Meng-kai Wang, Xuan He, Min Shi, Li-min Xie, Guo-xiang Jin, Hei-ying Gastroenterol Res Pract Research Article Aim. To gain insight into the molecular diversity of sapovirus in outpatients with acute gastroenteritis in Nanjing, China. Methods. The specimens from outpatients clinically diagnosed as acute gastroenteritis were detected by real-time PCR; RT-PCR was then performed to amplify part of VP1 sequences. The PCR products were cloned into pGEM-T Easy vector and bidirectionally sequenced. All sequences were edited and analyzed. A phylogenetic tree was drawn with the MEGA 5.0 software. Results. Between 2011 and 2013, 16 sapovirus positive cases were confirmed by real-time PCR. The infected cases increased from two in 2011 and six in 2012 to eight in 2013. The majority was children and the elderly (15, 93.75%) and single infections (15, 93.75%). Of the 16 real-time positive specimens, 14 specimens had PCR products and the analysis data of the 14 nucleic sequences showed that there was one GI genogroup with four genotypes, two GI.2 in 2011, three GI.2, and one GI.1 in 2012 and one GI.2, three GI.1, two GI.3, and two GI.5 in 2013. Conclusion. Our data confirmed continuous existing of GI genogroup and GI.2 genotype from 2011 to 2013 in Nanjing and the successive appearance of different genotypes from outpatients with gastroenteritis. Hindawi Publishing Corporation 2016 2016-08-31 /pmc/articles/PMC5021866/ /pubmed/27656204 http://dx.doi.org/10.1155/2016/4210462 Text en Copyright © 2016 Hong-ying Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Hong-ying Qiao, Meng-kai Wang, Xuan He, Min Shi, Li-min Xie, Guo-xiang Jin, Hei-ying Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title | Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title_full | Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title_fullStr | Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title_full_unstemmed | Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title_short | Molecular Diversity of Sapovirus Infection in Outpatients Living in Nanjing, China (2011–2013) |
title_sort | molecular diversity of sapovirus infection in outpatients living in nanjing, china (2011–2013) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021866/ https://www.ncbi.nlm.nih.gov/pubmed/27656204 http://dx.doi.org/10.1155/2016/4210462 |
work_keys_str_mv | AT zhanghongying moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT qiaomengkai moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT wangxuan moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT hemin moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT shilimin moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT xieguoxiang moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 AT jinheiying moleculardiversityofsapovirusinfectioninoutpatientslivinginnanjingchina20112013 |