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Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon
Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used for the efficient introduction of genetic modifications into ge...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021943/ https://www.ncbi.nlm.nih.gov/pubmed/27624004 http://dx.doi.org/10.1038/srep33225 |
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author | Li, Tianda Shuai, Ling Mao, Junjie Wang, Xuepeng Wang, Mei Zhang, Xinxin Wang, Leyun Li, Yanni Li, Wei Zhou, Qi |
author_facet | Li, Tianda Shuai, Ling Mao, Junjie Wang, Xuepeng Wang, Mei Zhang, Xinxin Wang, Leyun Li, Yanni Li, Wei Zhou, Qi |
author_sort | Li, Tianda |
collection | PubMed |
description | Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used for the efficient introduction of genetic modifications into genomes, and has already been successfully used to produce transgenic mice and rats. Here, we generated transgenic rats carrying either the desRed fluorescent protein (RFP) gene or the enhanced green fluorescent protein (eGFP) gene by injecting pronuclei with PB plasmids. We showed that the transgenic rats expressed the RFP or eGFP gene in many organs and had the capability to transmit the marker gene to the next generation through germline integration. In addition, rat embryonic stem cells (ESCs) carrying an RFP reporter gene can be derived from the blastocysts of the transgenic rats. Moreover, the RFP gene can be detected in chimeras derived from RFP ESCs via blastocyst injection. This work suggests that PB-mediated transgenesis is a powerful tool to generate transgenic rats expressing fluorescent proteins with high efficiency, and this technique can be used to derive rat ESCs expressing a reporter protein. |
format | Online Article Text |
id | pubmed-5021943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50219432016-09-20 Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon Li, Tianda Shuai, Ling Mao, Junjie Wang, Xuepeng Wang, Mei Zhang, Xinxin Wang, Leyun Li, Yanni Li, Wei Zhou, Qi Sci Rep Article Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential of embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used for the efficient introduction of genetic modifications into genomes, and has already been successfully used to produce transgenic mice and rats. Here, we generated transgenic rats carrying either the desRed fluorescent protein (RFP) gene or the enhanced green fluorescent protein (eGFP) gene by injecting pronuclei with PB plasmids. We showed that the transgenic rats expressed the RFP or eGFP gene in many organs and had the capability to transmit the marker gene to the next generation through germline integration. In addition, rat embryonic stem cells (ESCs) carrying an RFP reporter gene can be derived from the blastocysts of the transgenic rats. Moreover, the RFP gene can be detected in chimeras derived from RFP ESCs via blastocyst injection. This work suggests that PB-mediated transgenesis is a powerful tool to generate transgenic rats expressing fluorescent proteins with high efficiency, and this technique can be used to derive rat ESCs expressing a reporter protein. Nature Publishing Group 2016-09-14 /pmc/articles/PMC5021943/ /pubmed/27624004 http://dx.doi.org/10.1038/srep33225 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Li, Tianda Shuai, Ling Mao, Junjie Wang, Xuepeng Wang, Mei Zhang, Xinxin Wang, Leyun Li, Yanni Li, Wei Zhou, Qi Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title | Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title_full | Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title_fullStr | Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title_full_unstemmed | Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title_short | Efficient Production of Fluorescent Transgenic Rats using the piggyBac Transposon |
title_sort | efficient production of fluorescent transgenic rats using the piggybac transposon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021943/ https://www.ncbi.nlm.nih.gov/pubmed/27624004 http://dx.doi.org/10.1038/srep33225 |
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